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小木虫论坛-学术科研互动平台 » 计算模拟区 » 程序语言 » Shell&Perl » 讨论下怎么通过gi号批量获得物种的definition
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ldy2140

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[交流] 讨论下怎么通过gi号批量获得物种的definition 已有4人参与

做生物信息学的大都避免不了要blast 有时尽管我们blast出来的结果很多很吓人 但还是要将这些结果汇总成excel表格
最近就遇到了很让我头疼的事情 我做了很多转运蛋白的微生物全库的blast 但得到的table里只有匹配物种的gi号 在汇总结果的时候我想把gi号换成物种信息 比如像GBFF里的definition这种能说明物种遗传背景的字符串
所以我考虑用perl的正则表达式替换 写了如下的程序
CODE:
#!/usr/bin/perl
use Bio::Seq;
use Bio::DB::GenBank;

$gb = new Bio::DB::GenBank;
$^I = ".bak";

while (<>) {
  $line = $_;
  if ( /gi\|(\d+)\|/ ) {
    $gi = $1;
    $seq_obj = $gb->get_Seq_by_gi ($1);
    $def = $seq_obj->desc;
  }
  $_ = $line;
  s#\t.*?$gi.*?\t#\t$def\t#;
  print;
}

但是运行起来速度很慢而且很浪费带宽 因为用到的模块是将gi号对应的整个序列信息都下载下来 然后从中提取definition 所以效率很差 这是我花很短时间学习perl和bioperl编写的急功近利的程序 期待高手拍砖

[ Last edited by ldy2140 on 2012-8-28 at 21:55 ]
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1楼 2012-08-28 21:16:20
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libralibra

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你是不是有gi和description的对应关系,如果有,直接正则替换gi那部分即可.
如果必须去网络上查,查回来肯定gi序号和description同时有的,你处理完了再写文件
生物不懂,不过有bioperl,搜了下,也有biopython,照着例子改改,可以直接打印gi号和对应的description,可以看看.
准备学个脚本语言的时候,看过perl和python的语法,果断选了python,perl不懂啊
biopython教程:
http://biopython.org/DIST/docs/tutorial/Tutorial.html

例子代码,测试过了
CODE:
# Import the modules for interfacing with BLAST and parsing the output
from Bio.Blast import NCBIWWW, NCBIXML

# Blast the sequence of interest (in this case using the accession number
result_handle = NCBIWWW.qblast("blastn", "nr", "8332116")

# Parse the resulting output
blast_record = NCBIXML.read(result_handle)

# Loop over the alignments printing some output of interest
E_VALUE_THRESH = 0.004
for alignment in blast_record.alignments:
    result = alignment.title
    print 'gi no.: '+result.split()[0]
    print 'gi-desc: '+' '.join(result.split()[1:])
    print
##    for hsp in alignment.hsps:
##        if hsp.expect < E_VALUE_THRESH:
##            print
##            print '****Alignment****'
##            print 'sequence:', alignment.title
##            print 'length:', alignment.length
##            print 'e value:', hsp.expect
##            print hsp.query[0:75] + '...'
##            print hsp.match[0:75] + '...'
##            print hsp.sbjct[0:75] + '...'

结果,gi号和description可以分别提取打印:
CODE:
gi no.: gi|224094601|ref|XM_002310151.1|
gi-desc: Populus trichocarpa predicted protein, mRNA

gi no.: gi|359495761|ref|XM_002274845.2|
gi-desc: PREDICTED: Vitis vinifera uncharacterized LOC100267774 (LOC100267774), mRNA

gi no.: gi|349709091|emb|FQ378501.1|
gi-desc: Vitis vinifera clone SS0AEB13YG07

gi no.: gi|255562758|ref|XM_002522339.1|
gi-desc: Ricinus communis COR413-PM2, putative, mRNA

gi no.: gi|358346403|ref|XM_003637210.1|
gi-desc: Medicago truncatula Cold acclimation protein-like protein (MTR_079s1009) mRNA, complete cds

gi no.: gi|358344000|ref|XM_003636035.1|
gi-desc: Medicago truncatula Cold acclimation protein-like protein (MTR_026s0005) mRNA, complete cds

gi no.: gi|356561272|ref|XM_003548859.1|
gi-desc: PREDICTED: Glycine max uncharacterized protein LOC100817084 (LOC100817084), mRNA

gi no.: gi|356502211|ref|XM_003519866.1|
gi-desc: PREDICTED: Glycine max uncharacterized protein LOC100810337 (LOC100810337), mRNA

gi no.: gi|225311746|dbj|AK326681.1|
gi-desc: Solanum lycopersicum cDNA, clone: LEFL2011M15, HTC in fruit

gi no.: gi|255762732|gb|GQ370517.1|
gi-desc: Salvia miltiorrhiza cold acclimation protein (COR) mRNA, complete cds

gi no.: gi|225428595|ref|XM_002284686.1|
gi-desc: PREDICTED: Vitis vinifera uncharacterized LOC100248690 (LOC100248690), mRNA

gi no.: gi|297819785|ref|XM_002877730.1|
gi-desc: Arabidopsis lyrata subsp. lyrata COR413-PM2, mRNA

gi no.: gi|86755971|gb|DQ359747.1|
gi-desc: Chimonanthus praecox cold acclimation protein COR413-PM1 mRNA, complete cds

gi no.: gi|145339339|ref|NM_114943.4|
gi-desc: Arabidopsis thaliana cold-regulated 413-plasma membrane 2 (COR413-PM2) mRNA, complete cds

gi no.: gi|15810634|gb|AY056356.1|
gi-desc: Arabidopsis thaliana putative cold acclimation protein (At3g50830) mRNA, complete cds

gi no.: gi|10121842|gb|AF283005.1|
gi-desc: Arabidopsis thaliana cold acclimation protein WCOR413-like protein beta form mRNA, complete cds

gi no.: gi|13430785|gb|AF360305.1|
gi-desc: Arabidopsis thaliana putative cold acclimation protein (At3g50830) mRNA, complete cds

gi no.: gi|60317457|gb|AY761065.1|
gi-desc: Gossypium barbadense cold-related protein Cor413 (Cor413) mRNA, complete cds

gi no.: gi|255556172|ref|XM_002519075.1|
gi-desc: Ricinus communis COR413-PM2, putative, mRNA

gi no.: gi|156567558|gb|EU077497.1|
gi-desc: Poncirus trifoliata cold acclimation WCOR413-like protein mRNA, complete cds

gi no.: gi|46577795|gb|AY587773.1|
gi-desc: Tamarix androssowii putative stress-responsive protein mRNA, complete cds

gi no.: gi|305690597|gb|HQ010041.1|
gi-desc: Corylus heterophylla COR413-PM1 mRNA, complete cds

gi no.: gi|224105476|ref|XM_002313788.1|
gi-desc: Populus trichocarpa predicted protein, mRNA

gi no.: gi|242389633|emb|FP100664.1|
gi-desc: Phyllostachys edulis cDNA clone: bphylf036p06, full insert sequence

gi no.: gi|242382816|emb|FP092058.1|
gi-desc: Phyllostachys edulis cDNA clone: bphyem114p22, full insert sequence

gi no.: gi|242382391|emb|FP097178.1|
gi-desc: Phyllostachys edulis cDNA clone: bphylf028m11, full insert sequence

gi no.: gi|242381728|emb|FP091375.1|
gi-desc: Phyllostachys edulis cDNA clone: bphyst020e14, full insert sequence

gi no.: gi|238007351|gb|BT084358.1|
gi-desc: Zea mays full-length cDNA clone ZM_BFb0105L06 mRNA, complete cds

gi no.: gi|195636267|gb|EU965484.1|
gi-desc: Zea mays clone 286348 cold acclimation protein COR413-PM1 mRNA, complete cds

gi no.: gi|54652523|gb|BT017742.1|
gi-desc: Zea mays clone EL01N0449E04.c mRNA sequence

gi no.: gi|162459269|ref|NM_001111732.1|
gi-desc: Zea mays LOC542099 (gpm455), mRNA >gi|27902672|gb|AY181208.1| Zea mays cold acclimation protein COR413-PM1 mRNA, complete cds

gi no.: gi|21209119|gb|AY106041.1|
gi-desc: Zea mays PCO103483 mRNA sequence

gi no.: gi|242037992|ref|XM_002466346.1|
gi-desc: Sorghum bicolor hypothetical protein, mRNA

gi no.: gi|255617390|ref|XM_002539789.1|
gi-desc: Ricinus communis COR413-PM2, putative, mRNA

gi no.: gi|30690903|ref|NM_119885.2|
gi-desc: Arabidopsis thaliana cold acclimation protein WCOR413 (AT4G37220) mRNA, complete cds

gi no.: gi|26449888|dbj|AK117399.1|
gi-desc: Arabidopsis thaliana At4g37220 mRNA for putative ap2 cold acclimation protein, complete cds, clone: RAFL16-98-J01

gi no.: gi|226504237|ref|NM_001155133.1|
gi-desc: Zea mays cold acclimation protein COR413-PM1 (LOC100282221), mRNA >gi|195620729|gb|EU960077.1| Zea mays clone 221611 cold acclimation protein COR413-PM1 mRNA, complete cds

gi no.: gi|166359605|gb|EU365626.1|
gi-desc: Thellungiella halophila stress responsive protein (COR) mRNA, complete cds

gi no.: gi|150172175|emb|CU406592.1|
gi-desc: Oryza rufipogon (W1943) cDNA clone: ORW1943C102K01, full insert sequence

gi no.: gi|115455578|ref|NM_001057925.1|
gi-desc: Oryza sativa Japonica Group Os03g0767800 (Os03g0767800) mRNA, complete cds

gi no.: gi|10121844|gb|AF283006.1|
gi-desc: Oryza sativa (japonica cultivar-group) cold acclimation protein WCOR413-like protein mRNA, complete cds

gi no.: gi|32976054|dbj|AK066036.1|
gi-desc: Oryza sativa Japonica Group cDNA clone:J013049B03, full insert sequence

gi no.: gi|32970924|dbj|AK060906.1|
gi-desc: Oryza sativa Japonica Group cDNA clone:001-035-F05, full insert sequence

gi no.: gi|32970018|dbj|AK060000.1|
gi-desc: Oryza sativa Japonica Group cDNA clone:006-301-G09, full insert sequence

gi no.: gi|28973358|gb|BT005584.1|
gi-desc: Arabidopsis thaliana clone U50435 putative cold acclimation protein homolog (At4g37220) mRNA, complete cds

gi no.: gi|326534181|dbj|AK358227.1|
gi-desc: Hordeum vulgare subsp. vulgare mRNA for predicted protein, complete cds, clone: NIASHv1071H11

gi no.: gi|160954667|emb|CU225096.1|
gi-desc: Populus EST from leave

gi no.: gi|160950966|emb|CU229055.1|
gi-desc: Populus EST from severe drought-stressed leaves

gi no.: gi|357114154|ref|XR_137736.1|
gi-desc: PREDICTED: Brachypodium distachyon uncharacterized LOC100844112 (LOC100844112), miscRNA

gi no.: gi|224035946|gb|BT070152.1|
gi-desc: Zea mays full-length cDNA clone ZM_BFc0138N11 mRNA, complete cds

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4楼2012-08-29 17:25:33
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libralibra

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贴个例子看看

贴一个blast出来的结果(未处理的字符串)
贴一个你想要的结果(目标字符串)
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2楼2012-08-28 22:41:10
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ldy2140

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引用回帖:
2楼: Originally posted by libralibra at 2012-08-28 22:41:10
贴个例子看看

贴一个blast出来的结果(未处理的字符串)
贴一个你想要的结果(目标字符串)

sp|P23936|LACY_STRTR        gi|169822596|gb|ABJK02000022.1|        61.65        631        241        1        5        634        344705        342813        0.0         714
sp|P23936|LACY_STRTR        gi|223555729|gb|ACGH01000016.1|        57.01        628        260        1        2        619        65439        67322        0.0         692
替换后
sp|P23936|LACY_STRTR        Streptococcus infantarius subsp. infantarius ATCC BAA-102 S_infantarius-2.0.1_Cont245, whole genome shotgun sequence.        61.65        631        241        1        5        634        344705        342813        0.0         714
sp|P23936|LACY_STRTR        Lactobacillus buchneri ATCC 11577 contig00018, whole genome shotgun sequence.        57.01        628        260        1        2        619        65439        67322        0.0         692
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3楼2012-08-29 09:30:29
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wizardfan

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You know my comments on how to deal with high throughput data analysis: download the genbank flat file and parse the local file, which can improves the efficiency dramatically.

About your code:
1. Use "use strict;" all the time
2. Regular expression is fine, but I would use $` $' (special variables containing the previous and next part of the matching part) instead of another s/// statement
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5楼2012-08-29 22:54:33
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