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The higher efficiency of targeting when using SSR rather than RFLP probes is likely due to the greater specificity of PCR versus hybridization. The difference is also consistent with the report that RFLP probes hybridize, on average, to 2.55 map positions in the soybean genome (Shoemaker et al. 1996), and that the multiple fragments detected by RFLP frequently occur on different linkage groups (Keim et al. 1990). 真不好翻译啊,谁能翻译?谢谢! [search]翻译[/search] |
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