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love36183618木虫 (初入文坛)
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[求助]
论文摘要部分，大家帮帮忙，谢谢啦
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| DNA是生物的基本遗传物质,也是生命的物质基础.定量分析、特异性识别DNA分子,对基因学、分析生物学、病毒学等相关学科的发展具有非常重要的意义.目前,许多方法如荧光光谱法、紫外光谱法、电化学方法等都被用于DNA的研究.但是由于DNA分子自身的吸光,多采用荧光探针法检测DNA.荧光探针法与传统的方法相比,具有检测快速、操作简单、重复性好及辐射小等优点.在DNA自动测序、疾病诊断、临床医学等方面已经得到广泛的应用[5].有关用荧光光谱法研究DNA已经引起了人们的广泛关注.由于灵敏度是影响荧光探针检测的重要因素,因此找出更加灵敏的荧光探针已经成为当前研究的热点.用于核酸分析的荧光探针,目前有以下几类:有吖啶、菲啶类、荧光素、罗丹明类、菁类和金属配合物等.本文用自主发现的新型的BCDO荧光探针分子,采用荧光猝灭法,通过荧光光谱的测定研究了BCDO与DNA的相互作用,并对它们之间的作用方式进行了探讨。 |
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shuxinme111
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sltmac: 金币+30, 翻译EPI+1 2012-08-20 21:49:25
sltmac: 金币+30, 翻译EPI+1 2012-08-20 21:49:25
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DNA is not only the main hereditary substance of biological object, but also the material basis of life. Quantitative Analysis and specific recognition of DNA molecular play a significant role in the progress of related subjects like genetics, analytical biology and virology. Nowadays, many methods including Fluorescence Spectroscopy , ultraviolet spectroscopy, electrochemical technique have been applied in the DNA study. However, due to the self light-absorption of DNA molecular, Fluorescence Probe Method is used mostly to detect DNA. Compared with traditional methods, Fluorescence Probe Method is merit in fast detection, simple operation, good reproduction and little radiation, which has been widely used in automated DNA sequencing, disease diagnosis and clinical medicine. Therefore, it has attracted wide attention to study DNA by Fluorescence Spectroscopy method. Since sensitivity is an important factor to effect the detection by Fluorescence Probe, how to find out a more sensitive Fluorescence Probe becomes a research hotspot. Fluorescence Probes currently used for nucleic acid analysis can be classified to following kinds: acridines, phenanthridines, fluoreseeins, rhodamines, cyanines and metal complexes. In this paper, applied a newly self-found BCDO Fluorescence Probe molecular and fluorescence quenching method, the interaction and action mode between BCDO and DNA were studied and explored by detecting the and the fluorescence spectra. 供楼主参考～～～～ |
2楼2012-07-24 11:23:23