| 查看: 1800 | 回复: 13 | |||
[交流]
what a pity
|
|||
|
Together with other three person's samples, I ran the sequencing this morning. Unfortunately, no signal was collected. How can I explain to other three person. Because of me, they waited these data for 3 days, but now, they still can not get the results. How eager I am to solve the trouble, but I cannot. I have no idea about the bug of the machine. The only way for me now is to wait and ask technician for help tomorrow. Is it my bad luck leads to this? why the last person who used the machine got the data successfully?   |
回复此楼» 猜你喜欢
化学工程与技术324调剂
已经有8人回复
-
一志愿211电子信息347求调剂
已经有4人回复
-
材料与化工300求调剂
已经有20人回复
-
0856专硕求调剂 希望是a区院校
已经有8人回复
-
求调剂
已经有7人回复
-
本科西工大 0856 324求调剂
已经有9人回复
-
调剂
已经有8人回复
-
314求调剂
已经有12人回复
-
285求调剂
已经有19人回复
-
277 数一104，学硕，求调剂
已经有4人回复
fjtony163
版主 (文坛精英)
米米
- ESEPI: 7
- 应助: 361 (硕士)
- 贵宾: 6.876
- 金币: 70610.6
- 散金: 36050
- 红花: 222
- 沙发: 616
- 帖子: 21912
- 在线: 3793.7小时
- 虫号: 1397876
- 注册: 2011-09-11
- 专业: 遗传学研究新技术与方法
- 管辖: 论文翻译
10楼2012-07-16 16:22:15
★ ★ ★ ★ ★ ★ ★ ★
curton: 回帖置顶 2012-07-17 21:11:04
curton: 金币+8, Take it easy.Try again. you are the best 2012-07-17 21:11:33
curton: 回帖置顶 2012-07-17 21:11:04
curton: 金币+8, Take it easy.Try again. you are the best 2012-07-17 21:11:33
| Thank you very much, everyone. Now, I have known the reason. It is because of different version of Bigdye which leads to failure. The default setting of the Bigdye in the machine I used was version 3.1, but the Bigdye I used during PCR is version 1.1, therefore it can not distinguish the signal. It is my careless that induce this unnessary re-sequencing. |
14楼2012-07-17 14:25:55
★
小木虫: 沙发+1，金币+0.5, 恭喜抢个沙发，再给个红包
小木虫: 沙发+1，金币+0.5, 恭喜抢个沙发，再给个红包
 |
2楼2012-07-16 15:55:19
★
小木虫: 金币+0.2, 抢了个小板凳，给个红包
小木虫: 金币+0.2, 抢了个小板凳，给个红包
|
|
3楼2012-07-16 15:55:46
|
|
4楼2012-07-16 15:56:33
|
|
5楼2012-07-16 15:56:46
|
|
6楼2012-07-16 15:56:56
|
|
7楼2012-07-16 15:58:30
|
|
8楼2012-07-16 15:58:40
Justonestand
木虫 (著名写手)
- ESEPI: 11
- 应助: 23 (小学生)
- 贵宾: 0.039
- 金币: 2358.8
- 散金: 210
- 红花: 20
- 沙发: 17
- 帖子: 1499
- 在线: 238小时
- 虫号: 1865037
- 注册: 2012-06-20
- 性别: MM
- 专业: 金属非晶态、准晶和纳米晶
9楼2012-07-16 16:05:26
