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In this study, we used partial T-cell depletion and low-dose irradiation with a hematopoietic cell infusion and a very brief course of immunosuppression to produce donor HSC engraftment in the recipient before allotransplanting skin from the hematopoietic cell donor. Both primarily vascularized skin flaps and secondarily vascularized conventional skin grafts were allotransplanted. These 2 modes of skin transplantation were used because previous work has suggested that the mode of transplantation may affect the susceptibility of skin to rejection.3 This has been supplemented by work we have carried out (Horner et al, in press, JPRAS) that indicates there are significant differences in the immune response depending on the method of skin transplantation.

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