你们都说用培养基分装好,那请问是加了10%FBS的DMEM-F12培养基呢?还是直接没有加FBS的培养基呢?说明书上说
0.1% BSA!可是不好无菌操作啊?
我用的是PEPROTECH公司的Recombinant Human FGF-basic说明书上说For extended storage, it is
recommended to further dilute in a buffer containing a
carrier protein (example 0.1% BSA) and store in working!
Catalog# 100-18B
Lot # 031208