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共享：英文版Two Hybrid System-Methods and Protocols
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已搜无重复，不知道是不是搜漏了 Two-Hybrid Systems Methods and Protocols Edited by Paul N. MacDonald 强烈要求，喜欢的请支持下 Preface........................................................................................... v Contributors .................................................................................. xi PART I GENERAL METHODS 1 The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel .............................................. 3 2 Growth and Maintenance of Yeast Lawrence W. Bergman ................................................................ 9 3 Media Formulations for Various Two-Hybrid Systems Michael Saghbini, Denise Hoekstra, and Jim Gautsch ........ 15 4 Yeast Two-Hybrid Vectors and Strains Philip James ............................................................................... 41 5 High-Efficiency Transformation of Plasmid DNA into Yeast Robin A. Woods and R. Daniel Gietz ...................................... 85 6 Qualitative and Quantitative Assessment of Interactions Monica M. Montano ................................................................... 99 7 Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies Alyson Byrd and René St-Arnaud ......................................... 107 PART II FALSE POSITIVES 8 Two-Hybrid System and False Positives: Approaches to Detection and Elimination Ilya G. Serebriiskii and Erica A. Golemis ............................. 123 9 Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns Dennis M. Kraichely and Paul N. MacDonald ...................... 135 10 Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones Louie Naumovski ..................................................................... 151 Contents PART III APPLICATIONS 11 Smad Interactors in Bone Morphogenetic Protein Signaling Xiangli Yang and Xu Cao ........................................................ 163 12 Protein Interactions Important in Eukaryotic Translation Initiation Katsura Asano and Alan G. Hinnebusch.............................. 179 13 Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins Sergio A. Oñate........................................................................ 199 14 Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-apoptosis Protein E1B-19K Thirugnana Subramanian and G. Chinnadurai.................... 211 PART IV ALTERNATIVE STRATEGIES 15 Mammalian Two-Hybrid Assays: Analyzing Protein-Protein Interactions in the Transforming Growth Factor-β Signaling Pathway Xin-Hua Feng and Rik Derynck.............................................. 221 16 One-Hybrid Systems for Detecting Protein-DNA Interactions Mary Kate Alexander, Brenda D. Bourns, and Virginia A. Zakian ......................................................... 241 17 The Split-Hybrid System: Uncoding Multiprotein Networks and Defining Mutations That Affect Protein Interactions Phyllis S. Goldman, Anthony J. DeMaggio, Richard H. Goodman, and Merl F. Hoekstra .................... 261 18 Three-Hybrid Screens: Inducible Third-Party Systems Björn Sandrock, Franck Tirode, and Jean-Marc Egly......... 271 19 Three-Hybrid Screens for RNA-Binding Proteins: Proteins Binding 3' End of Histone mRNA Zbigniew Dominski and William F. Marzluff......................... 291 20 Membrane Recruitment Systems for Analysis of Protein–Protein Interactions Ami Aronheim .......................................................................... 319 Index ..........................................................................................329 南河小生 邮件已发出请查收! [ Last edited by telomerase on 2007-5-7 at 00:30 ] |
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