[交流] 黄酮类药物的酶法修饰。

3楼: Originally posted by xysi at 2012-05-27 00:05:06
Most of the flavonoids or flavones have poor aqueous solubility. You can try the enzymatic modification in organic solvents. For this, you may begin with reading the recently published book 'enzymes  ...

8楼: Originally posted by magnolia414 at 2012-05-28 07:48:30
可以将黄酮溶于醇或DMSO，再加入水相中，有机相的比例尽量不要超过0.2％

9楼: Originally posted by 红魔亮亮 at 2012-05-28 19:59:23
谢谢，是不是担心有机试剂使酶失活。如果在10%的有机溶剂中，依然能保持一定的酶活，可以增大有机溶剂的比例吗？...

10楼: Originally posted by magnolia414 at 2012-05-29 06:44:57
是这样，一般的酶都不耐有机溶剂。0.2%是个保守量，绝大部分酶在这个条件下都不受影响，当然每个酶的耐受上限是有差异的。如果你的酶在10％的有机相中仍然能保持活性，那么加大有机相的比例应该没有问题。另外样品 ...