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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ 感谢参与,应助指数 +1 wenlongli82: 金币+50, ★★★★★最佳答案, 谢谢 2012-05-26 10:01:53
Callose deposition at plasmodesmata is a critical factor in restricting the cell-to-cell movement of Soybean mosaic virus
作者: Li, WL (Li, Wenlong)1; Zhao, YS (Zhao, Yongshan)1; Liu, CJ (Liu, Chunji)2; Yao, GB (Yao, Guibin)1; Wu, SS (Wu, Sisi)1; Hou, CY (Hou, Chunyan)1; Zhang, MC (Zhang, Mengchen)3; Wang, DM (Wang, Dongmei)1
来源出版物: PLANT CELL REPORTS 卷: 31 期: 5 页: 905-916 DOI: 10.1007/s00299-011-1211-y 出版年: MAY 2012
被引频次: 0 (来自 Web of Science)
引用的参考文献: 46 [ 查看 Related Records ] 引证关系图
摘要: Callose is a beta-l,3-glucan with diverse roles in the viral pathogenesis of plants. It is widely believed that the deposition of callose and hypersensitive reaction (HR) are critical defence responses of host plants against viral infection. However, the sequence of these two events and their resistance mechanisms are unclear. By exploiting a point inoculation approach combined with aniline blue staining, immuno-electron microscopy and external sphincters staining with tannic acid, we systematically investigated the possible roles of callose deposition during viral infection in soybean. In the incompatible combination, callose deposition at the plasmodesmata (PD) was clearly visible at the sites of inoculation but viral RNA of coat protein (CP-RNA) was not detected by RT-PCR in the leaf above the inoculated one (the upper leaf). In the compatible combination, however, callose deposition at PD was not detected at the site of infection but the viral CP-RNA was detected by RT-PCR in the upper leaf. We also found that in the incompatible combination the fluorescence due to callose formation at the inoculation point disappeared following the injection of 2-deoxy-d-glucose (DDG, an inhibitor of callose synthesis). At same time, in the incompatible combination, necrosis was observed and the viral CP-RNA was detected by RT-PCR in the upper leaf and HR characteristics were evident at the inoculation sites. These results show that, during the defensive response of soybean to viral infection, callose deposition at PD is mainly responsible for restricting the movement of the virus between cells and it occurs prior to the HR response.
入藏号: WOS:000302813600011
文献类型: Article
语种: English
作者关键词: Callose; Plasmodesmata; Soybean; Soybean mosaic virus; Hypersensitive reaction
KeyWords Plus: BEAN-LEAVES; PLANTS; TOBACCO; ARABIDOPSIS; TRANSPORT; LESIONS; BETA-1,3-GLUCANASE; LOCALIZATION; EXPRESSION; RESISTANCE
通讯作者地址: Wang, DM (通讯作者),Agr Univ Hebei, Coll Life Sci, Lekainanda St 2596, Baoding 071001, Hebei, Peoples R China
地址:
1. Agr Univ Hebei, Coll Life Sci, Baoding 071001, Hebei, Peoples R China
2. CSIRO Plant Ind, St Lucia, Qld 4067, Australia
3. Hebei Food & Oil Crops Inst, Shijiazhuang 050031, Hebei, Peoples R China
电子邮件地址: dongmeiwang63@hotmail.com
基金资助致谢:
基金资助机构 授权号
National Natural Science Foundation of China 30971706
Natural Science Foundation of Hebei Province C2008000321
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We thank Dr. Haijian Zhi (University of Nan Jing Agricultural University, China) for providing the virus strains used and advice on their propagation. We thank Shanjin Huang (Institute of Botany, the Chinese Academy of Sciences, China) for reading the manuscript and providing helpful comments. This work was supported by the National Natural Science Foundation of China (no. 30971706) and by the Natural Science Foundation of Hebei Province (no. C2008000321).
出版商: SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA
Web of Science 分类: Plant Sciences
学科类别: Plant Sciences
IDS 号: 926EH
ISSN: 0721-7714 |
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