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Çë´ó¼Ò°ïæ²éһϣ¬ÎÄÕÂEfficient plant regeneration from cotyledonary node
explants of Cucumis melo L.
Huijun Zhang
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,Gao Peng
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and Luan Feishi*
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Efficient plant regeneration from cotyledonary node explants of Cucumis melo L.
×÷Õß: Zhang, HJ (Zhang, Huijun)1; Peng, G (Peng, Gao)1; Feishi, L (Feishi, Luan)1
À´Ô´³ö°æÎï: AFRICAN JOURNAL OF BIOTECHNOLOGY  ¾í: 10   ÆÚ: 35   Ò³: 6757-6761   ³ö°æÄê: JUL 13 2011
±»ÒýƵ´Î: 0 (À´×Ô Web of Science)
ÒýÓõIJο¼ÎÄÏ×: 17 [ ²é¿´ Related Records ]     ÒýÖ¤¹ØÏµÍ¼ÒýÖ¤¹ØÏµÍ¼     
ÕªÒª: An efficient regeneration system was developed using cotyledonary node of two inbred line of melon, Cucumis melo 'CM-15' and 'CM-23'. The induction of shoots was achieved on Murashige and Skoog (MS) solid medium supplemented with different combinations of 6-benzylaminopurine (BA) and indole-3-acetic acid (IAA). The best shoot induction was observed when the explants were cultured on MS medium supplemented with BA (1.5 mg/L) and IAA (0.1 mg/L), and on MS medium supplemented with BA (1.5 mg/L) and IAA (0.5 mg/L) for 'CM-15' and 'CM-23', and inbred line CM-15 had the best regeneration rate. The shoot clumps were transferred to MS with BA (0.05 mg/L), resulting to the differentiation of most of the shoots initially into well developed shoots. Regenerated shoots were rooted on half-strength MS medium without plant growth regulators (PGRs). The rooted plants were established in soil with 100% success rate. This high frequency plant regeneration system provides improved technology to assist in genetic transformation of melon.
Èë²ØºÅ: WOS:000294668200011
ÎÄÏ×ÀàÐÍ: Article
ÓïÖÖ: English
×÷Õ߹ؼü´Ê: Cucumis Melo L.; cotyledonary node; shoots regeneration; plant regeneration
KeyWords Plus: AGROBACTERIUM-MEDIATED TRANSFORMATION; SOMATIC EMBRYOGENESIS; IN-VITRO; ORGANOGENESIS; MAAZOUN
ͨѶ×÷ÕßµØÖ·: Feishi, L (ͨѶ×÷Õß),NE Agr Univ, Hort Coll, Harbin 150030, Heilongjiang Pr, Peoples R China
µØÖ·:
1. NE Agr Univ, Hort Coll, Harbin 150030, Heilongjiang Pr, Peoples R China
µç×ÓÓʼþµØÖ·: luanfeishi@sina.com
»ù½ð×ÊÖúÖÂл:
»ù½ð×ÊÖú»ú¹¹        ÊÚȨºÅ
National Natural Science Foundation of China        
31071812
30871723
China Agriculture Research System        
CARS-26-02
[ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢]   
³ö°æÉÌ: ACADEMIC JOURNALS, P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA
Web of Science ·ÖÀà: Biotechnology & Applied Microbiology
ѧ¿ÆÀà±ð: Biotechnology & Applied Microbiology
IDS ºÅ: 817JL
ISSN: 1684-5315
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2Â¥2012-05-11 10:24:26
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zhhuijun: ½ð±Ò+1 2012-05-13 06:55:06
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×÷Õß: Zhang, HJ (Zhang, Huijun)1; Peng, G (Peng, Gao)1; Feishi, L (Feishi, Luan)1
À´Ô´³ö°æÎï: AFRICAN JOURNAL OF BIOTECHNOLOGY  ¾í: 10   ÆÚ: 35   Ò³: 6757-6761   ³ö°æÄê: JUL 13 2011
±»ÒýƵ´Î: 0 (À´×Ô Web of Science)
ÒýÓõIJο¼ÎÄÏ×: 17 [ ²é¿´ Related Records ]     ÒýÖ¤¹ØÏµÍ¼ÒýÖ¤¹ØÏµÍ¼     
ÕªÒª: An efficient regeneration system was developed using cotyledonary node of two inbred line of melon, Cucumis melo 'CM-15' and 'CM-23'. The induction of shoots was achieved on Murashige and Skoog (MS) solid medium supplemented with different combinations of 6-benzylaminopurine (BA) and indole-3-acetic acid (IAA). The best shoot induction was observed when the explants were cultured on MS medium supplemented with BA (1.5 mg/L) and IAA (0.1 mg/L), and on MS medium supplemented with BA (1.5 mg/L) and IAA (0.5 mg/L) for 'CM-15' and 'CM-23', and inbred line CM-15 had the best regeneration rate. The shoot clumps were transferred to MS with BA (0.05 mg/L), resulting to the differentiation of most of the shoots initially into well developed shoots. Regenerated shoots were rooted on half-strength MS medium without plant growth regulators (PGRs). The rooted plants were established in soil with 100% success rate. This high frequency plant regeneration system provides improved technology to assist in genetic transformation of melon.
Èë²ØºÅ: WOS:000294668200011
ÎÄÏ×ÀàÐÍ: Article
ÓïÖÖ: English
×÷Õ߹ؼü´Ê: Cucumis Melo L.; cotyledonary node; shoots regeneration; plant regeneration
KeyWords Plus: AGROBACTERIUM-MEDIATED TRANSFORMATION; SOMATIC EMBRYOGENESIS; IN-VITRO; ORGANOGENESIS; MAAZOUN
ͨѶ×÷ÕßµØÖ·: Feishi, L (ͨѶ×÷Õß),NE Agr Univ, Hort Coll, Harbin 150030, Heilongjiang Pr, Peoples R China
µØÖ·:
1. NE Agr Univ, Hort Coll, Harbin 150030, Heilongjiang Pr, Peoples R China
µç×ÓÓʼþµØÖ·: luanfeishi@sina.com
»ù½ð×ÊÖúÖÂл:
»ù½ð×ÊÖú»ú¹¹        ÊÚȨºÅ
National Natural Science Foundation of China         
31071812
30871723
China Agriculture Research System         
CARS-26-02
[ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢]   
³ö°æÉÌ: ACADEMIC JOURNALS, P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA
Web of Science ·ÖÀà: Biotechnology & Applied Microbiology
ѧ¿ÆÀà±ð: Biotechnology & Applied Microbiology
IDS ºÅ: 817JL
ISSN: 1684-5315
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3Â¥2012-05-11 10:43:12
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