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【答案】应助回帖
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lina8804: 金币+50, 翻译EPI+1, ★★★很有帮助 2012-05-03 09:20:01
3.5 The influence of reaction time to system fluorescence
Fixing other conditions ,we inspected the fluorescence quench of system of BCDO-DNA in 2,5,10,15,20,25,30 minutes ,and the results figured in 3.12 and 3.13 .
According to the figure of 3.12 and 3.13 ,we draw the fluorescence quench intensity with the changing of time ,which showed in figure 3.14 .
According to the figure of 3.14 ,the system of fluorescence quench value would be balance after 5min of putting into DNA .We knew that the DNA of calf and BCDO would react completely after 5min of putting into HMTA-HCl .Therefore ,we let the system settled 5-10min after putting into DNA ,and then we detected the system .
3.6 The influence of solvent to the system fluorescence
As a result of BCDO didn’t solve in water ,we set ethanol as solvent .We settled the concentration of BCDO and DNA ,and acidity of buffer solution ,then we inspected the fluorescence quench of system of BCDO-DNA in 2,5,10,15,20,25,30 % ,and the results figured in 3.15 and 3.16 .
According to the figure of 3.15 and 3.16 ,we draw the fluorescence quench intensity with the changing of concentration ,which showed in figure 3.17 .
According to the figure of 3.17 ,when the content of ethanol under 10% ,it had little influence in system ,but it had a serious effect with the increase of ethanol .
3.7 The influence of temperature to the system fluorescence
Fixing other conditions ,we inspected the fluorescence quench of system of BCDO-DNA in 20,25,30,35,40,45,50,60℃ ,and the results figured in 3.18 and 3.19 .
According to the figure of 3.18 and 3.19 ,we draw the fluorescence quench intensity with the changing of temperature ,which showed in figure 3.20 .The result just measured in room temperature . |
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