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2 Experimental Reagent grade aniline (Sigma-Aldrich) was distilled under reduced pressure prior to use. Deoxyribonucleic acid (DNA) was purchased from Tokyo Chemical Industry. All other chemicals were of analytical grade and were used asreceived. The aqueous solutions were prepared using distilled and deionized water. The polymerizing solution for PANI was an aqueous solution containing 0.5 M (1 M = 1 mol dm-3) CF3COOH and 50 mM aniline. The reason why CF3COOH was employed as an acid was the low growth rate of PANI. It is widely accepted that the growth rate is dependent on the type of acid in the polymerizing solution, although the reason has not yet been elucidated [21–24]. The lowest growth rate is observed for CF3COOH [23, 24]. It is likely that the lower the growth rate becomes, the more favorably PANI molecules are rearranged for effective incorporation of DNA during the electropolymerization. A standard three-electrode cell was employed, comprising a working electrode, a commercial Ag/AgCl electrode and a Pt plate counter electrode with an electrode area of about 8 cm2. Three working electrodes were used for each purpose: platinum wire electrodes with an electrode area of 0.296 cm2 for the cyclic voltammogram (CV) measurements; platinum plate electrodes for the morphology observations of the obtained PANI films; and optically transparent electrodes (ITO) for the measurements of the absorption spectra of the PANI films. Prior to use, the platinum wire and plate electrodes were treated with aqua regia for 30 s and then polarized by repeated potential cycling between -0.2 and 1.2 V vs. Ag/AgCl in 0.1 M H2SO4 until a voltammogram showed features associated with hydrogen adsorption/desorption and oxide formation/ removal [25]. The electrochemical experiments were carried out using a Hokuto Denko HSV-100 potentiostat connected to a personal computer. The morphology of the obtained PANI was measured using a JEOL JSM-6320F field-emission scanning electron microscope (SEM). The PANI samples were dried under vacuum for 24 h at room temperature after adequate rinsing with water. A 5 nm Pt layer was sputtered on the samples prior to the SEM measurements. |
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Mally89: 金币+1 2012-05-07 17:06:02
sltmac: 金币+5, 翻译EPI+1 2012-05-14 08:27:03
Mally89: 金币+1 2012-05-07 17:06:02
sltmac: 金币+5, 翻译EPI+1 2012-05-14 08:27:03
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实验 试剂级苯胺(Sigma-Aldrich)使用前减压蒸馏。DNA从日本化工公司购买。所有其它试剂都是试剂级的。水溶液由蒸馏水和去离子水制备。PANI 水溶液包含0.5 M(1 M = 1 mol dm-3) CF3COOH and 50 mM苯胺。之所以用CF3COOH是由于在PANI水溶液中变化较慢。二聚体溶液的增长率取决于酸的强度，尽管原因未知。CF3COOH的增长速率最慢。在电聚合过程中，低增长速率有利于DNA分子的合成。一个标准的三电子电解池由一个商业的Ag/AgCl电极和Pt电极区域大约8cm2。三个工作电极用途各异： 无线电的电荷区域大约 0.296 cm2 (CV)，镀铂的电极用于获得PANI薄膜，光转化电极(ITO)用于测定PANI薄膜的吸收谱 。使用前铂线和铂电极要用王水处理30秒，然后用 -0.2 and 1.2 V vs. Ag/AgCl in 0.1 M H2SO4电池极化，直到紫外下有氢吸收和氧的转移。 [25]. 电化学实验用Hokuto Denko HSV-100 仪器与一个个人电脑直接相连。获得的PANI形态学监测使用JEOL JSM-6320F 电微波扫场 (SEM)。除水后，将PANI样品在室温下真空干燥24小时. 在SEM测定时向样品上涂一5nm厚的铂层。 |
2楼2012-04-25 18:49:23