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Analysis. Aliquots of saturated sodium 4-nitrobenzenesulphonate solutions were transferred into a tarred volumetric flask. The concentration of sodium 4-nitrobenzenesulphonate in aqueous solutions was determined using a Shimadzu-6A high-performance liquid phase chromatograph (HPLC). The chromatographic column used was a unimicro Kromasil C18, 5 (250mm × 4.6mm) maintained at 308.2 K. The HPLC system consisted of a Shimadzu SPD-6A UV single wavelength spectrophotometric detector was set to 270 nm. The mobile phase consists of three eluents which are water, Na2SO4 and H3PO4. The concentration ranges from (0 to 1.5) mgmL-1 of analytes were used for construction of calibration curves. Each analysis was repeated three times, and the average value of the three measurements was considered as the final value of the analysis. The uncertainty of the experimental solubility values is about 2.0 %. The uncertainty in the solubility can be due to uncertainties in the temperature measurements and weighing procedure. |
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至尊木虫 (职业作家)
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-轩影鳶: 金币+12, 翻译EPI+1, ★★★很有帮助 2012-04-24 14:30:05
-轩影鳶: 金币+12, 翻译EPI+1, ★★★很有帮助 2012-04-24 14:30:05
| 分析。4－硝基苯磺酸钠的饱合水溶液转移到定容烧瓶中。用HPLC检测4－硝基苯磺酸钠在水溶液中的浓度。用C18色谱柱，维持温度在308.2K。HPLC系统由SHIMADZU SPD-6A组成， 紫外单一波长设定在270nm处。流动相由水，Na2SO4和H3PO4组成。浓度从0到1.5mg/ml分别进行分析，制成曲线。每一个分析重复三次，平均值作为最后分析值。实验误差大约在2%左右。实验的不确定性主要由实验室温度和称量引起。 |
2楼2012-04-24 14:21:10