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[求助]
微生物方面一句话
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| 请问这句话什么意思Polyclonal OmcS antibodies were raised against the purified OmcS protein in rabbits (NewEngland Peptide, Gardner, MA). The third-bleed crude antiserum was incubated with membrane-blotted OmcS (100g) and washed with TBST buffer (20 mM Tris-HCl [pH7.5], 0.5 M NaCl, and 0.05% Tween 20). |
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gywyl1977
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2楼2012-04-19 12:24:03
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是这方面的，我不是做这方面的，但是现在要看这篇文章，不是很明白。下面还有几句麻烦顺便翻译下。不甚感激。 The purified OmcS antibodies were tested for specificity using Western blot analysis (One-Step Western complete kit with tetramethyl benzidine [TMB]; GenScript) of total proteins prepared from cell lysates of the G. sulfurreducens wild type and the mutant strain in which the gene encoding OmcS had been deleted. Total proteins (10 g) were separated by 10% SDS-PAGE, blotted onto membranes using a semidry transfer cell (Bio-Rad), and incubated with the purified OmcS antibodies. Only one band with a molecular mass corresponding to that of the OmcS protein (i.e., 50 kDa) was detected with Western blot analysis in the wild-type cell lysate, whereas no bands were detected in the cell lysate from the OmcS deletion mutant (data not shown) |
3楼2012-04-19 19:13:16