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Abstract. A spectrophotometric and derivative spectrophotometric study of
Cu-dithizonate complex in aqueous phase in the presence of Triton X-100, a
neutral surfactant, is reported. The system obeys Beer's law between 1.0 • 10 -6-
9.0 • 10 -6 mol/1 of Cu2+; detection limit is 12 ng/ml. The molar absorption
coefficient, specific absorptivity and Sandell's sensitivity of the complex are
3.06 • 104 1 mo1-1 cm -1, 0.4825 ml g-1 cm-1 and 2.1 • 10 -3 pg cm -2, respectively.
The conditional stability constant of the 1 : 2 complex, calculated considering
simultaneously existing equilibria, has been found to be 1.73 • 1011 12
mo1-2 (I = 0.07, pH 1.4, temperature = 10~ Absorption studies in the derivative
mode have been carried out to determine the absorption maximum of
the complex and to overcome interference due to the presence of certain metal
ions. The method has been validated by determination of copper in beers, wines,
human hair, goat liver and fly ash samples.
Key words: dithizone, copper dithizonate, derivative spectrophotometric determination
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