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huangminghe金虫 (初入文坛)
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[求助]
关于利用旋转圆盘电极测量动力学参数问题
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我利用旋转圆盘电极研究P450酶对药物的催化，我首先固定药物的浓度，测量了不同转速下的催化电流，得到了J−1 vs ω−1/2 的线性关系，我想利用 Koutecky− Levich equations 计算 the number of electrons transferred (n) and J K can be obtained from the slope and intercept of the Koutecky−Levich plots。但是这个公式中的扩散系数和运动粘度我不知道是多少，有没有人知道这些参数我应该怎么查到或者通过什么计算方法可以得到，我用的电解质是0.1M pH=7.4 的磷酸盐缓冲溶液，药物选择的是硝苯地平，酶是CYP3A4。请理解的给我一些建议，谢谢！ |
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4楼2012-04-17 08:55:24