| 查看: 2184 | 回复: 1 | ||
| 【奖励】 本帖被评价1次，作者shinevip增加金币 0.5 个 | ||
| 当前主题已经存档。 | ||
[资源]
Holtfreter's Solution 显微注射鱼受精卵的保存液
|
||
|
本人搜索过整个小木虫，还没有转基因鱼受精卵的保存液Holtfreter's Solution 的配方。 下面是它的配方链接，适用于不同阶段的鱼卵配方都有。 http://www.msu.edu/user/eisthen/ ... malcare/holtfr.html |
回复此楼» 猜你喜欢
体制内长辈说体制内绝大部分一辈子在底层，如同你们一样大部分普通教师忙且收入低
已经有8人回复
-
过年走亲戚时感受到了所开私家车的鄙视链
已经有9人回复
-
今年春晚有几个节目很不错，点赞！
已经有10人回复
-
情人节自我反思：在爱情中有过遗憾吗？
已经有10人回复
-
基金正文30页指的是报告正文还是整个申请书
已经有5人回复
★★★ 三星级,支持鼓励
|
Holtfreter's Solution -------------------------------------------------------------------------------- Source: based on Armstrong, J.B., Duhon, S.T., and Malacinski, G.M. (1989) Raising the axolotl in captivity. In J.B. Armstrong and G.M. Malacinski (eds.) Developmental Biology of the Axolotl. New York: Oxford University Press, pp. 220-227. Modified protocol (but not recipe) 2/26/98 when we realized that MgSO4 and CaCl2 easily combine and precipitate out. -------------------------------------------------------------------------------- 100% Holtfreter's solution for dejellied embryos, before stage 38 NaCl 3.5 g NaHCO3 0.2 g KCl 0.05 g MgSO4 stock solution 333 µl CaCl2 stock solution 333 µl dH20 1 liter Check pH; should be between 7 and 7.5. 50% Holtfreter's solution for older embryos, larvae, and adults flask carboy trash can NaCl 1.75 g 35 g 262.5 g NaHCO3 0.1 g 2 g 15 g KCl 0.025 g 0.5 g 3.75 g MgSO4 stock solution 335 µl 6.66 ml 50 ml CaCl2 stock solution 335 µl 6.66 ml 50 ml Novaqua 335 µl 6.66 ml 50 ml dH20 1 liter 20 liters 150 liters Check pH; should be between 7 and 7.5. -------------------------------------------------------------------------------- MgSO4 stock solution Combine: MgSO4 300 g deionized water 500 ml CaCl2 stock solution Combine: CaCl2 150 g deionized water 500 ml -------------------------------------------------------------------------------- |
2楼2007-04-02 22:50:01