| 查看: 2242 | 回复: 1 | ||
| 【奖励】 本帖被评价1次，作者shinevip增加金币 0.5 个 | ||
| 当前主题已经存档。 | ||
[资源]
Holtfreter's Solution 显微注射鱼受精卵的保存液
|
||
|
本人搜索过整个小木虫，还没有转基因鱼受精卵的保存液Holtfreter's Solution 的配方。 下面是它的配方链接，适用于不同阶段的鱼卵配方都有。 http://www.msu.edu/user/eisthen/ ... malcare/holtfr.html |
回复此楼» 猜你喜欢
大龄残疾硕士的一点执念
已经有19人回复
-
今年E04面上
已经有19人回复
-
2026年面上项目中了，2A+B, 会评顺利通过
已经有10人回复
-
刑法学论文投稿求助
已经有5人回复
-
Journal of Environmental Chemical Engineering
已经有3人回复
-
最后一年，祈求好运
已经有3人回复
-
风电环氧领域
已经有3人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有4人回复
-
植酸TLC薄层色谱爬板
已经有6人回复
★★★ 三星级,支持鼓励
|
Holtfreter's Solution -------------------------------------------------------------------------------- Source: based on Armstrong, J.B., Duhon, S.T., and Malacinski, G.M. (1989) Raising the axolotl in captivity. In J.B. Armstrong and G.M. Malacinski (eds.) Developmental Biology of the Axolotl. New York: Oxford University Press, pp. 220-227. Modified protocol (but not recipe) 2/26/98 when we realized that MgSO4 and CaCl2 easily combine and precipitate out. -------------------------------------------------------------------------------- 100% Holtfreter's solution for dejellied embryos, before stage 38 NaCl 3.5 g NaHCO3 0.2 g KCl 0.05 g MgSO4 stock solution 333 µl CaCl2 stock solution 333 µl dH20 1 liter Check pH; should be between 7 and 7.5. 50% Holtfreter's solution for older embryos, larvae, and adults flask carboy trash can NaCl 1.75 g 35 g 262.5 g NaHCO3 0.1 g 2 g 15 g KCl 0.025 g 0.5 g 3.75 g MgSO4 stock solution 335 µl 6.66 ml 50 ml CaCl2 stock solution 335 µl 6.66 ml 50 ml Novaqua 335 µl 6.66 ml 50 ml dH20 1 liter 20 liters 150 liters Check pH; should be between 7 and 7.5. -------------------------------------------------------------------------------- MgSO4 stock solution Combine: MgSO4 300 g deionized water 500 ml CaCl2 stock solution Combine: CaCl2 150 g deionized water 500 ml -------------------------------------------------------------------------------- |
2楼2007-04-02 22:50:01