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danyzj01

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[求助] 1%溴酚蓝是如何配制的,为什么在水里溶解不完全?

这样过滤器后浓度低了如何用于配制上样缓冲液?
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yjchsh

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Dr.

【答案】应助回帖

感谢参与,应助指数 +1
加几滴5M的NaOH很快就溶解了
在配置SDS样品处理液时用的是pH6.8的Tris-HCl缓冲液
所以没有影响
2楼2012-04-18 11:24:20
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danyzj01

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引用回帖:
2楼: Originally posted by yjchsh at 2012-04-18 11:24:20:
加几滴5M的NaOH很快就溶解了
在配置SDS样品处理液时用的是pH6.8的Tris-HCl缓冲液
所以没有影响

如果用乙醇溶解可行吗?用百分之多少的比较好?
3楼2012-04-18 20:32:13
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yjchsh

铁杆木虫 (正式写手)

Dr.

【答案】应助回帖

Of course, please look at the following information from sigma

Bromophenol Blue
B0126 Sigma-Aldrich

solubility

NaOH: soluble (The water-soluble sodium salt was prepared by grinding the powder in a mortar with equimolecular amounts of N/10-NaOH, i.e. about 6 ml. for each 400 mg powder.)  
   ethanol: soluble (A stock solution of BPB was prepared by dissolving 134 mg BPB in 20mL 96% ethanol and diluted to 100mL with water. 3)
4楼2012-04-19 01:24:25
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danyzj01

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4楼: Originally posted by yjchsh at 2012-04-19 01:24:25:
Of course, please look at the following information from sigma

Bromophenol Blue
B0126 Sigma-Aldrich

solubility

NaOH: soluble (The water-soluble sodium salt was prepared by grinding the  ...

不过乙醇密度较低,到时候上蛋白样的时候会不会整个浮起来
5楼2012-04-19 20:21:34
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yjchsh

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Dr.

【答案】应助回帖

★ ★ ★ ★
danyzj01: 金币+4, ★★★★★最佳答案 2012-04-21 09:14:19
引用回帖:
5楼: Originally posted by danyzj01 at 2012-04-19 20:21:34:
不过乙醇密度较低,到时候上蛋白样的时候会不会整个浮起来

That not gonna happen. Because there is pretty much glycerol in your sample.
Do not worry about it.
Why don't your use NaOH to solubilize the Bromophenol Blue?
But using ethanol is also fine.
6楼2012-04-20 01:01:31
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