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weantony

铜虫 (正式写手)

[求助] 关于microRNA的引物设计问题

如题 我想测血中microRNA的含量,准备使用real-time pcr,现在需要设计cdna扩增的引物,我想请问我是需要按照microRNA的序列设计还是按照microRNA的前体的序列设计吖 我刚开始接触分子生物学的实验 不是太懂 谢谢各位指教拉
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hushengli1

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【答案】应助回帖

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weantony: 金币+5, ★有帮助 2013-01-16 12:43:28
你应该先根据microRNA的序列设计反转录引物,再根据你反转录得到的cDNA的序列设计定量引物。
2楼2013-01-14 16:48:20
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