| 查看: 552 | 回复: 1 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
devilpanda金虫 (小有名气)
|
[求助]
小弟求助
|
||
|
Whole-cell extracts were prepared by lysing the cells with RIPA buffer containing 150 mM NaCl, 50 mM Tris HCl (pH 8), 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor and phosphatase inhibitor cocktails (Sigma- Aldrich). The cell extracts were separated by 10% SDS-PAGE and transferred onto a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA). Samples were incubated in blocking buffer (0.1% Tween 20 and 5% nonfat milk powder in Tris-buffered saline [TBS]) for 1 h at room temperature. Afterward, the membrane was incubated with primary antibody in blocking buffer overnight at 4 °C before being washed twice with TBST (0.1% Tween in TBS) and incubated with the appropriate secondary antibody in blocking buffer for 1 h at room temperature. The blot was developed using ECL Western blotting substrate (Millipore) and analyzed using a luminescent image analyzer (LAS-4000 mini; FujiFilm, Tokyo, Japan). The primary antibodies were used at the following dilutions: rat anti-ABCG2, 1:100 (Abcam, Cambridge, UK); rabbit anti-PARP, 1:1000 (Cell Signaling Technology, Beverly, MA, USA); rabbit anti-caspase 7, 1:1000 (Cell Signaling Technology); rabbit anti-Akt and phosphate Akt-ser 473, 1:1000 (Cell Signaling Technology); and mouse anti-β-actin, 1:10000 (Sigma-Aldrich)谁能帮我翻译下啊~~  |
回复此楼» 猜你喜欢
283求调剂
已经有20人回复
-
求调剂
已经有10人回复
-
材料工程302分求调剂
已经有17人回复
-
一志愿华中农微生物，288分，三年实验经历
已经有10人回复
-
化学工程与技术324调剂
已经有22人回复
-
273求调剂
已经有51人回复
-
材料复试求调剂
已经有18人回复
-
一志愿211，化学学硕，310分，本科重点双非，求调剂
已经有24人回复
-
调剂
已经有9人回复
-
生物学调剂 可调剂到生物与医药
已经有6人回复
懒蛋
铁杆木虫 (著名写手)
- 翻译EPI: 8
- 应助: 28 (小学生)
- 金币: 7107.7
- 散金: 318
- 红花: 9
- 沙发: 1
- 帖子: 1809
- 在线: 356.5小时
- 虫号: 1231871
- 注册: 2011-03-13
- 性别: GG
- 专业: 环境微生物学
2楼2012-04-18 04:40:15
