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其他条件对体系荧光的影响 由于在强电解质NaCl的存在下，往往使DNA的构象发生改变，从而影响DNA的结合作用，因此在较佳实验条件下，考察Na+的浓度和变性DNA对体系荧光的影响。确定NaCl浓度在0～0.01mol•L-1范围内时，Na+对BCDO-DNA体系的影响不大。变性DNA对BCDO的荧光光谱几乎没有影响，也就是变性DNA与BCDO基本上没有作用。变性DNA可能由于双螺旋结构的解旋，与BCDO不能够进行相互作用。据此推断BCDO与DNA作用的方式应该与DNA的双螺旋结构有关。 |
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yuanbing0000
至尊木虫 (小有名气)
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sltmac: 金币+1 2012-04-10 08:57:39
sltmac: 金币+1 2012-04-10 08:57:39
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供参考： The system fluorescent is affected by other factors Strong electrolyte NaCl being, the conformation of DNA will sometimes be transformed and the combination of DNA will be affected. Therefore, under preferable experimental conditions, Na+ concentration and denatured DNA impact on system fluorescent is reviewed. Within the Na+ concentration range of 0～0.01mol•L-1, it is certain that Na+ exerts not too much effects on BCDO-DNA system and denatured DNA exerts little effects on fluorescent spectrum of BCDO, namely, there is mainly no chemical action between denatured DNA and BCDO for the separation of twin-spiral structure of denatured DNA. On these grounds, chemical action manners between BCD and DNA are related to twin-spiral structure of denatured DNA.. |
» 本帖已获得的红花（最新10朵）
lina88042楼2012-04-09 19:11:15
爱朝霞
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3楼2012-04-09 21:06:20
爱朝霞
金虫 (小有名气)
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sltmac: 金币+1 2012-04-10 08:57:47
lina8804: 金币+10, 翻译EPI+1, ★★★很有帮助 2012-04-17 15:39:06
sltmac: 金币+1 2012-04-10 08:57:47
lina8804: 金币+10, 翻译EPI+1, ★★★很有帮助 2012-04-17 15:39:06
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仅供参考Other factors affecting the system fluorescent The existence of NaCl always makes the conformation of DNA change and the combination effect of DNA will also be affected. Therefore in a preferable exprrimental condition the effects of the concentration of Na+ and denatured DNA on system fluorescent are investigated. When the concentration of the NaCl is in the range of 0-0.01moi/L Na+ has slight effect on BCDO-DNA system and denatured DNA has little effect on the fluorescent spectrum of BCDO, which indicates that denatured DNA has no effects on BCDO. For the unwinding of the duplex of the DNA it can not interact with BCDO. According to this the way of the interaction between BCDO and DNA is related to the duplex of the DNA. |
4楼2012-04-09 21:19:52
5楼2012-04-17 15:40:33
谢啦！ 6楼2012-04-17 15:41:15
