|
|
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
Mally89: 金币+3, 感谢应助！~欢迎常来！~吼吼！~\(^o^)/~ 2012-03-30 22:42:30
sltmac: 金币+50 2012-04-16 18:13:49
sltmac: 金币+50 2012-04-16 18:13:54
sltmac: 金币+50 2012-04-16 18:14:02
sltmac: 金币+50 2012-04-16 18:14:07
sltmac: 金币+50 2012-04-16 18:14:13
sltmac: 金币+30, 翻译EPI+1 2012-04-16 18:14:19
Objective:
Peripheral blood and synovial tissue of CIA rats knee synovial tissue and peripheral blood, as the main object of study, the pathological changes of the joint tissue, joint points in the peripheral blood of IL-1, IL-6 and SOD in two in the level of MDA.GSH-Px content in synovial tissue vascular endothelial growth factor (VEGF) receptor FLT-4 and of FLK was-1, and hypoxia-inducible factor-1α (HIF-1α), cytokines changes for the major indexes, and more than Tripterygium glycosides as control Qubi side of the RA treatment, and reveal the mechanism of RA from the effects of treatment, in order to find more effective treatment for the treatment of RA programs.
Methods:
Selection of clean grade healthy male SD rats 72, one week after the feeding of the adaptability of randomly selected 12 as the normal control group, the remaining 60 of the base of the tail subcutaneous injection of type Ⅱ collagen and Freund's complete adjuvant-induced arthritis model, specific methods for: the proper amount of C II solution (concentration 2mg/ml) was added dropwise to equal volume of incomplete Freund's adjuvant, C II, a final concentration of 1mg/ml. Ice bath using emulsified with homogenizer for the degree of non-proliferation, to the dropping water emulsified in the mixture according to 0.2ml / only 200μg of C Ⅱ / only, subcutaneous injection in the base of the tail. After 7 days, 0.1ml /, subcutaneous 100μg C II / only at the base of the tail, strengthen the immune once the 10 days can be induced arthritis model. 15d after immunization, 60 of CIA rats were randomly divided into four groups: high dose group of the CIA model group, Tripterygium Glycosides group, Qubi square Qubi side low dose group, 15 in each group. And begin gavage once daily for four consecutive w. Each group administration as follows: Qubi side high dose group: 6.66 g / kg • d; Qubi side low-dose group: 3.33 g / kg • d; Tripterygium glycosides: 9.68 mg / kg • d; normal control the size of the group and CIA model rats fed with pure water. The animals were observed arthritis index, joint swelling. Animals were sacrificed after four weeks of treatment, under the light microscope to observe the pathology of the rat ankle injury; ELISA method for the determination of the anti-II collagen antibody in animal serum, IL-1, IL-6 levels; Determination of serum SOD the two vascular endothelial growth factor (VEGF) receptor FLT-4, MDA and GSH-Px in activity; by immunohistochemical method to detect knee joint synovial tissue and of FLK was 1, and hypoxia-inducible factor-1α (HIF-1α), cytokine changes.
Results:
Joint injury results showed that: compared with normal control group, 15 d after immunization, rat arthritis index was significantly increased (P <0.01), each model rat arthritis index was no significant difference. Compared with model group, after administration of Tripterygium glycosides group and Qubi of side group rat arthritis index decreased, but not statistically significant. CIA model rats swollen joints was significantly higher than the normal control group (P <0.01); compared with the CIA model group, Tripterygium Glycosides group, high-dose rats Qubi side joint swelling decreased significantly (P <0.01 ), high dose Qubi side joint swelling degree of the rats was significantly lower than Tripterygium glycosides group (P <0.05)
Light microscope visible visible synovial cell hyperplasia, disorganized, and synovial tissue congestion and edema, capillary proliferation, and visible inflammatory cell infiltration; villous proliferation of synovial tissue formation, and can stretch the depths of the joint cavity, or to cartilage surface crawling to the formation of pannus. Under the cover of the pannus cartilage surface can be seen clearly the organization of the cartilage surface degeneration and necrosis. The articular cartilage surface exfoliation, the intra-articular joint cartilage and synovial tissue off the peel.
After treatment, proliferation of synovial cells to reduce congestion and edema of the synovial tissue is significantly reduced, reduced the number of vascular proliferation and infiltration of inflammatory cells, pannus formation were significantly reduced. Articular cartilage surface of a small amount of visible flat layer exfoliation, stripping the formation of cartilage, subchondral bone, trabecular bone size, arrangement basically normal. Compared with model group, high dose group of Qubi side and joint damage of Tripterygium glycosides rats significantly reduced (P <0.01, P <0.05); Qubi side high dose group compared with Tripterygium glycosides group, pathological integral decreased significantly (P <0.05)
The serum levels of cytokines measured by ELISA results show that: compared with the normal control group, the CIA model of serum anti-type II collagen antibodies, IL-1β, IL-6 levels were significantly increased; Tripterygium glycosides group and cured Bi side high-dose group, serum anti-type II collagen antibody, IL-1β, IL-6 levels are significantly lower than in model group; Qubi side low-dose group, serum anti-type II collagen antibody levels in the CIA model group showed no significant difference. Tripterygium glycosides, anti-type II collagen antibody Qubi side high dose group, IL-1β levels decreased significantly Qubi side low-dose group, serum IL-1β levels were significantly decreased (P <0.05), while IL -6 levels were significantly decreased, but not statistically significant.
Of GSH-Px, SOD and MDA activity in the colorimetric assay of serum showed that: compared with normal control group, the CIA model group, serum GSH-Px in the activity of SOD decreased significantly (P <0.05, P <0.01), MDA, activity was significantly increased (P <0.01). Tripterygium glycosides and high and low dose Qubi party can make the serum GSH-Px activity was significantly increased MDA activity decreased significantly (P <0.05, P <0.01), Tripterygium Glycosides and the dose Qubi side also SOD activity was significantly increased (P <0.01).
Two were detected by immunohistochemistry in synovial tissue vascular endothelial growth factor (VEGF) receptor FLT-4 and of FLK was-1, the results showed that: compared with the normal control group, model group, serum levels of FLK-1, FLT- 4 levels were significantly increased (P <0.01), high dose group of Tripterygium glycosides group Qubi side serum level of FLK-1 is significantly lower than in model group (P <0.05, P <0.01); Qubi side high-dose group, serum FLK-1 compared with Tripterygium glycosides group was significantly reduced, Tripterygium Glycosides group and Qubi side high dose group, serum level of FLK-1 compared with the model group was significantly lower (P <0.01, P < 0.05); Qubi side low dose group, serum FLK-1, of FLT-4 levels were significantly decreased, but not statistically significant.
Hypoxia-inducible factor-1α (HIF-1α) test results showed that: compared with normal control group, model group, serum levels of HIF-1α levels were significantly increased (P <0.01), while Tripterygium glycosides and Qubi side high-dose group, serum levels of HIF-1α levels than in model group decreased significantly (P <0.01); remove low dose of the paralysis side serum of HIF-1α levels were significantly decreased, but not statistically significant.
Conclusion:
Eradicates paralysis side can significantly reduce the CIA rat ankle joint swelling degree and arthritis index has obvious therapeutic effect on CIA rats, its efficacy is superior to the Tripterygium glycosides.
Qubi side can significantly reduce serum anti-type II collagen antibody levels, reduce the formation of immune complexes, inhibition of CIA rats with abnormal autoimmune response, and lowered serum IL-1, IL-6, MDA, increase GSH-Px and SOD levels, modulation of immune status to improve synovitis lesions, improving the metabolism of oxygen free radicals, reduce bone destruction, so as to achieve the role of treatment of the CIA.
Eradicates paralysis can be lower in synovial tissue vascular endothelial growth factor (VEGF) in the two receptors FLT-4 and FLK-1 and HIF-1α in content, thus inhibiting angiogenesis and synovial cell proliferation, and improve joint synovitis lesions, treatment of the CIA's role. |
|
回复此楼