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Intensity Distribution
The fi rst order result from a DLS experiment is an intensity distribution of
particle sizes. The intensity distribution is naturally weighted according to
the scattering intensity of each particle fraction or family. For biological
materials or polymers the particle scattering intensity is proportional to
the square of the molecular weight. As such, the intensity distribution
can be somewhat misleading, in that a small amount of aggregation/
agglomeration or presence or a larger particle species can dominate the
distribution. However this distribution can be used as a sensitive detector
for the presence of large material in the sample.
2Â¥2012-03-17 22:25:10
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Volume Distribution
Although the fundamental size distribution generated by DLS is an
intensity distribution, this can be converted, using Mie theory, to a
volume distribution or a distribution describing the relative proportion of
multiple components in the sample based on their mass or volume rather
than based on their scattering (Intensity.)
When transforming an intensity distribution to a volume/mass
distribution, there are 4 assumptions that must be accepted.
• All particles are spherical
• All particles are homogeneous
• The optical properties of the particles are known, i.e. the real &
imaginary components of the refractive index
• There is no error in the intensity distribution
3Â¥2012-03-17 22:26:00
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