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For the assessment of adhesion processes at a level that mimics the in vivo state, isolated primary cells have been used. For example, cells that were isolated from zebrafish embryos were used to examine the importance of non-canonical Wnt signaling for cell adhesion in early development, and the authors used SCFS to determine the specific adhesion of different types of primary cells to functionalized substrates as well as to other cells (Puech et al., 2005; Ulrich et al., 2005). Moreover, using the same system, the specific contributions of cell adhesion versus cell-cortex tension to cell sorting during zebrafish gastrulation have been clarified (Krieg et al., 2008). |
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至尊木虫 (知名作家)
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Mally89: 金币+1, 感谢应助！~欢迎常来！~吼吼！~\(^o^)/~ 2012-03-18 09:31:50
lhyhappy8888: 金币+15, 翻译EPI+1 2012-03-18 10:55:26
Mally89: 金币+1, 感谢应助！~欢迎常来！~吼吼！~\(^o^)/~ 2012-03-18 09:31:50
lhyhappy8888: 金币+15, 翻译EPI+1 2012-03-18 10:55:26
| 为了在模拟体内状态的水平上评估粘附过程，使用分离的原代细胞。比如，使用从斑马鱼胚胎中分离出的细胞来检查非权威的Wnt信号对于早起发育中细胞粘连的重要性，作者使用SCFS 来测定不同类型原代细胞与功能化底物和其他细胞的特异性粘附 (Puech et al., 2005; Ulrich et al., 2005)。另外，用同样的系统，阐明了斑马鱼原肠胚形成过程中细胞粘附而非细胞-皮层张力对细胞排架的特异性贡献。 |
2楼2012-03-18 00:54:36