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【答案】应助回帖
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canfeng2008(金币+20, 翻译EPI+1): ★有帮助 我还得再改改，不过还是谢谢你了 2012-03-11 01:28:26
sltmac: 金币+20 2012-03-22 09:46:21
供参考：
The peroxisome proliferator-activated receptor γ (PPARγ) plays a key role in the process of lipid metabolism by regulating the expression of target genes of lipid metabolism and promoting lipid deposition. However, PPARγ’s function in in promoting lipid deposition is inhibited when it is combined with the FoxO1, a subfamily of the forkhead transcription factors. By far, a great much has been reported on the research on fish lipid deposition from the perspectives of nutrition, biochemistry, and physiology, but rare research has been done on the molecular and protein levels, especially on the molecular mechanism of mutual regulation between two genes. Therefore, the grass carp, a unique type of cyprinid fish in China, is to be chosen as the test material in this project, and such tissue, cellular, proteomic, and molecular biotechnologies as recombinant protein expression, Western blotting, cell culture, immunocytochemistry, chromatin immunoprecipitation, and real-time quantitative PCR, etc. are employed to study the reaction mechanism between FoxO1 and PPARγ, filter out the respective target genes, and explore the regulatory networks and molecular mechanisms in the regulation of lipid metabolism of grass carp. The research results not only shed light on the clarification of the molecular mechanisms for lipid metabolism of China’s fish, but also provide a theoretical basis for improving the flavor and quality of fish. |
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