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liuxus20030704

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[交流] 求助外文中的一个实验操作

For flavonoids (F) determination, skin extract and wine samples were diluted 100 times, with ethanol-hydrochloric acid solution and an absorbance spectrum in the range 230-700 nm was recorded. The F content was calculated according to the following formula:
F=E280*df*82.4
E280 = specific extinction coefficient at 280nm assessed with the graphic method (absorbance corresponding to the segment, parallel to the y-axis, starting from the peak maximum at 280 nm and finishing on the tangent joining the points of minimum at the left and at the right of the peak).

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liuxus20030704(金币+1):谢谢参与
tuobaohua(金币+1): 积极参与交流,欢迎常来科研版! 2012-02-29 20:54:23
3楼2012-02-29 19:37:22
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liuxus20030704

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longwave(金币+1): 鼓励交流,欢迎常来科研版! 2012-02-29 18:59:29
在一篇文献中看到测试类黄酮的方法,请问the graphic method 中是如何得到E280 specific extinction coefficient ?不知道怎么画图和计算?急用,谢谢! 图片搞忘传了。
2楼2012-02-29 17:13:15
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