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求助外文中的一个实验操作
For flavonoids (F) determination, skin extract and wine samples were diluted 100 times, with ethanol-hydrochloric acid solution and an absorbance spectrum in the range 230-700 nm was recorded. The F content was calculated according to the following formula:
F=E280*df*82.4
E280 = specific extinction coefficient at 280nm assessed with the graphic method (absorbance corresponding to the segment, parallel to the y-axis, starting from the peak maximum at 280 nm and finishing on the tangent joining the points of minimum at the left and at the right of the peak). |
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