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炮打总统府金虫 (小有名气)
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[求助]
求助环境工程水处理方向翻译！
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A 1 L Erlenmeyer flask was equipped with an oxygen electrode connected to a recorder. The solution for the SOUR test for ammonium oxidizers composed of 100 mg N/L ammonium and other minerals without any organic compound or nitrite. The solution for nitrite oxidizers composed of 100 mg N/L nitrite and other minerals without organic compound or ammonium. A control test contained only minerals. A mixed liquor sample or a detached-biofilm suspension was saturated with oxygen by shaking and transferred to the flask.The initial biomass concentration in the batch reactors was in the range of 700–1000 mg VSS/L for suspended growth cells and 150–300 mg VSS/L for detached biofilm cells. The flask was carefully closed without leaving air bubbles. For the attached cell experiment, the detachment of the biofilm from the surface of the media collected from the reactor was performed using a vortex (MO Bio Vortex) for 5 min at the maximum speed and by adding polyox-yethylene lauryl ether to aid in removing cells from surfaces and promoting their lyses . This procedure was repeated three times. The degree of detachment was monitored to determine the suitability of the method by measuring microbial activity remaining in the medium by adenosine triphosphate (ATP) and no activity was observed in the medium (data not shown).The cell suspension was stirred with a magnetic stirrer at 150 rpm, 30 8C and pH 8, respectively. The SOUR (mg O 2/g VSS d) of each sample was equal to the slope of DO depletion versus time divided by the VSS in the flask. |
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【答案】应助回帖
炮打总统府(金币+20, 翻译EPI+1): ★★★很有帮助 2012-02-17 17:21:42
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如下，仅供参考。polyox-yethylene lauryl ether 试剂名称未能译出。。。 在一只1升的锥形瓶中装入连有记录器的氧电极。在SOUR测试中，用于测试氨氧化细菌的溶液含100 mg N/L 的氨和其他矿物质，且不含任何有机成分或亚硝酸盐。用于测试亚硝酸盐氧化细菌的溶液中含100 mg N/L 亚硝酸盐其他矿物质，且不含任何有机成分或氨。对照试验（的溶液中）只含矿物质。通过摇晃使混合试液或分离生物膜的悬浮液中的溶解氧达到饱和，然后将其转移到该锥形瓶中。序批式反应器中含有的初始生物量浓度为：700–1000 mg VSS/L 悬浮污泥生物量 和 150–300 mg VSS/L 的分离生物膜生物量。小心地将锥形瓶封闭起来，确保瓶中没有残留气泡。 在附带的细胞试验中， 将从反应器中收集的生物膜媒质放入漩涡（分离器）来分离表面的生物膜。分离转速设为最大，分离时间为5分钟。 并加入polyox-yethylene lauryl ether 来帮助细胞从表面分离并促进细胞溶解 。该过程重复三次。为了检测生物膜分离的程度和衡量该方法的适用性，本实验采用adenosine triphosphate (ATP)方法检测媒质上剩余的生物活性。 检测结果显示媒质不再具有生物活性（数据未显示）。细胞悬浮液用磁力搅拌器在150 rpm的速度下搅拌，液体温度30.8C，pH 值为8。各种试液中的SOUR等于溶解氧消耗随时间变化（曲线）的斜率除以锥形瓶中的VSS。 |
2楼2012-02-17 15:00:15
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