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炮打总统府金虫 (小有名气)
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For the enumeration of ammonium or nitrite oxidizers, ready-to-use detection kits for nitrifying bacteria were used: Immuno latex Kensyutukun (Yakult Central Institute for Microbiological Research, Yakult Honsha Co. Ltd.)Polyclonal antibodies for ammonium oxidizers and nitrite oxidizers combined with latex particles were provided as reagents in this kit. Binary serial dilutions were added into the wells of a 96-well microtiter plate, and the antibody-latex reagent was dropped into the samples.After incubation for 15 h at room temperature, ammonium and nitrite oxidizers were detected by latex coagulations. The cell numbers were achieved by the following equation concentration of ammonium oxidizer or nitrite oxidizers) = (maximum dilution times in which coagulation can be detected) · (titer value of the antibody provided by the kit). |
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【答案】应助回帖
炮打总统府(金币+10, 翻译EPI+1): ★有帮助 2012-02-19 18:26:55
sltmac(金币+10): 2012-02-26 11:04:51
sltmac(金币+10): 2012-02-26 11:04:51
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使用硝化细菌检测试剂盒– Immuno latex Kensyutukun (Yakult Honsha公司Yakult 微生物研究中心)来计算氨氧化细菌或亚硝酸氧化细菌的数量。该试剂盒中的反应试剂由氨氧化细菌和亚硝酸氧化细菌的多克隆抗体与混合乳胶颗粒物合成。在一个96孔微孔板中加入二倍系列稀释的水样，并滴入抗体-乳胶反应试剂。经15 小时室温培养后可以通过观察乳胶絮凝物（的生成）检测氨氧化细菌和亚硝酸氧化细菌。细胞数量可由以下公式计算： 氨氧化细菌或亚硝酸氧化细菌的浓度=絮凝能够产生时的（水样）最大稀释倍数×试剂盒中的抗体的滴定值 |
2楼2012-02-18 00:48:42
