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炮打总统府金虫 (小有名气)
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[求助]
求助环境工程水处理方向的一段英文翻译，谢谢！
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For the enumeration of ammonium or nitrite oxidizers, ready-to-use detection kits for nitrifying bacteria were used: Immuno latex Kensyutukun (YakultCentral Institute for Microbiological Research, Yakult Honsha Co. Ltd.) .Polyclonal antibodies for ammonium oxidizers and nitrite oxidizers combined with latex particles were provided as reagents in this kit. Binary serial dilutions were added into the wells of a 96-well microtiter plate, and the antibody-latex reagent was dropped into the samples. After incubation for 15 h at room temperature, ammonium and nitrite oxidizers were detected by latex coagulations. The cell numbers were achieved by the following equation: (concentration of ammonium oxidizer or nitrite oxidizers)=(maximum dilution times in which coagulation can be detected) x (titer value of the antibody provided by the kit). 2.5. Specific oxyReu uptake rate (SOUR) A 1 L Erlenmeyer flask was equipped with an oxygen electrode to a recorder.The solution for the SOUR test for ammonium connected oxidizers composed of 100 mg N/L ammonium and other minerals without any organic compound or nitrite. The solution for nitrite oxidizers composed of 100 mg N/L nitrite and other minerals without organic compound or ammonium. A control test contained only minerals. A mixed liquor sample or a detached-biofihn suspension was saturated with oxygen by shaking and transferred to the flask The initial biomass concentration in the batch reactors was in the range of 700-1000 mg VSS/L for suspended growth cells and 150-300 mg VSS/L for detached biofihn cells. The flask was carefully closed without leaving air bubbles. For the attached cell experiment, the detachment of the biofihn from the surface of the media collected from the reactor was performed using a vortex (MO Bio Vortex) for 5 min at the maximum speed and by adding polyoxyethylene lauryl ether to aid in removing cells from surfaces and promoting their lyses . This procedure was repeated three times. The degree of detachment was monitored to determine the suitability of the method by measuring microbial activity remaining in the medium by adenosine tripho-sphate (ATP) and no activity was observed in the medium (data not shown).The cell suspension was stirred with a magnetic stirrer at 150 rpm, 30 C and pH 8, respectively. The SOUR (mg OZ/g VSS d) of each sample was equal to the slope of DO depletion versus time divided by the VSS in the flask. |
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