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qianlinguang金虫 (正式写手)
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Isolation and Characterization of Four Forms of Kallikrein from Hog Pancreas Autolysate1 Four forms of kallikrein, designated as I-IV, were isolated from hog pancreas autolysate mainly by chromatographies on Lysine-aminohexyl-Sepharose and on DEAE-Sepharose CL-6B, with yields of 65, 56, 59, and 41 mg, respectively, from 10 kg of the tissue. They were homogeneous on polyacrylamide gel electrophoresis, indistinguishable from eath other immunologically and had the same amino acid composition. Kallikreins I, II, and III contained carbohydrate, but kallikrein IV was essentially carbohydrate free. On reduction with mercaptoethanol, each of them produced two polypeptide chains with different molecular weights. The H (heavy) chains from kallikreins I and II were identical, designated as Hj. It was a glycoprotein with apparent molecular weight of 21,000, whereas the H chain from III and IV, designated as H,, had a molecular weight of 17,000 and was regarded as an H! chain devoid of carbohydrate. Likewise, the L (light) chain from kallikreins I and III, designated as LL, was a glycoprotein with an apparent molecular weight of 12,000, whereas L, from II and IV corresponded to L^ devoid of carbohydrate and had a molecular weight of 8,500. Thus, kallikreins I-IV could be expressed as Hil^, HiL,, HJLJ, and H,Li, respectively. They had comparable specific activities and Km values towards synthetic substrates. The isoelectric points of kallikreins I-IV were nearly the same, with values of 4.0-4.1. |
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wenglixing
银虫 (正式写手)
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【答案】应助回帖
qianlinguang(金币+10, 翻译EPI+1): ★★★很有帮助 谢谢哦 2012-02-02 12:41:35
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从猪胰腺Autolysate1四种形式激肽释放酶的分离和表征 四种形式激肽释放酶I - IV，主要是从猪胰腺autolysate中分离aminohexyl-赖氨酸- Sepharose和DEAE-琼脂糖凝胶CL- 6B层析，从10公斤的组织分别获得65，56，59和41毫克。他们在聚丙烯酰胺凝胶电泳中均质，其他免疫无异，并有相同的氨基酸组成。激肽释放酶Ⅰ，Ⅱ，Ⅲ包含碳水化合物，但激肽释放酶IV是基本上无碳水化合物。减少巯基乙醇，他们产生了两种不同分子量的多肽链。激肽释放酶I和II的H（重型）链是一样的，称为Hj。它是一种糖蛋白，具有明显的分子量为21,000，而III和IV链，称为H，H链分子量为17000，是缺乏碳水化合物的H链。同样，链激肽释放酶I和III的L（轻）链，称为LL，是糖蛋白，其分子量12000，而从II和IV的L，对应于无碳水化合物L链，分子量为8500 。因此，激肽释放酶I - IV可分别表示为 Hil，HiL，HJLJ，和HLi。他们对合成的底物有比较专一的活性和Km值。激肽释放酶的I - IV的等电点几乎是相同的，在4.0-4.1之间。 |
2楼2012-02-01 17:56:45
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