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[求助]
求帮忙翻译一段摘要啊，中译英，谢谢了
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| 采用PCR-RFLP方法分析了鲫鱼不同品系mtDNA ND3/ND4L/ND4基因片段，对野鲫、异育银鲫、红鲫、白鲫、彩鲫、金鱼的基因片段进行测序，测序结果用RESearch-version1.0软件对限制性内切酶特异性位点进行分析，最终选择MvaI核酸限制性内切酶对目的基因片段进行酶切，同时引入鲤鱼作为外类群,构建了系统树。我们对多个样本进行酶切结果分析表明：基于聚合酶链式反应—限制性片段长度多态性分析能快速而准确地区分鲫鱼的四个不同品种，为物种鉴定提供了一定的理论研究基础，但野鲫、金鱼、彩鲫的基因序列两两只有一个碱基的差异，相似度接近100%，在酶切软件290种酶分析结果中未能找到区别三者的限制性内切酶位点，这一结果也验证了科研工作者的许多关于金鱼、彩鲫鱼起源于野鲫的观点。 |
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terry_well
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爱与雨下(金币+2): 2011-12-05 08:47:48
sltmac(金币+30, 翻译EPI+1): 2011-12-18 10:14:59
爱与雨下(金币+2): 2011-12-05 08:47:48
sltmac(金币+30, 翻译EPI+1): 2011-12-18 10:14:59
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Different strains of mtDNA ND3/ND4L/ND4 gene fragments for different carps were analyzed by means of PCR-RFLP, in which the gene fragments of wild carp, crucian carp, red carp, crucian carp and color crucian carp and goldfish were sequenced. The sequence results were analyzed by RESearch-version 1.0 software, and the specific restriction enzyme sites was calculated. MvaI DNA was finally selected as a restriction endonuclease to digest the fragments of the gene, at the same time, carp was introduced as an outgroup to construct a phylogenetic tree. It was shown from our analysis to the multiple samples that we could distinguish the four different varieties of carp rapidly and accurately based on polymerase chain reaction namely analysis of restriction fragment length polymorphism, which provided a theoretical basis for further study on species identification. However, only one base difference between two kinds among wild carp, goldfish and carp color for their gene sequences, which means their similarity close to 100%. Besides, it was failed to find any restriction enzyme sites point based on the analysis results of about 290 kinds of enzymes in the digestion software, which also verified a number of viewpoints from research workers on the origination of goldfish and color carp. 翻译的不好，多多谅解，仅供参考。  |
2楼2011-12-05 03:19:58
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