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linyiming2新虫 (初入文坛)
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[求助]
SALSA MLPA kit P208 Human Telomere-6-紧急，求大侠相助
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This SALSA MLPA probemix can also be bought as part of the Telomere Follow-up Set. The Telomere Follow-up Set covers all subetelomeres and contains 12 Telomere follow-up probemixes: 465 telomere probes divided over 46 regions. The introduction price (valid in 2011) for 12 ?? 25 reactions is ? 1200. This P208-C1 probemix can be used to confirm and further characterise abnormalities detected by SALSA MLPA kit P036 and/or SALSA MLPA kit P069 / P070 used for broad human subtelomere screening. This SALSA® MLPA® P208-C1 Human Telomere-6 probemix contains: 11 probes in the terminal 3.7 Mb of chromosome arm 2p 10 probes in the terminal 4.4 Mb of chromosome arm 3p 11 probes in the terminal 5.9 Mb of chromosome arm 6p 13 probes in the terminal 6.4 Mb of chromosome arm 8p SALSA MLPA kits P036 and P069 / P070 each contain one MLPA probe for each subtelomeric region. Most of these probes detect a well-characterized gene close to the telomere. The probes in P036 and P069 / P070 are different, allowing results obtained with one probemix to be confirmed by the other. The majority of probes in P069 and P070 are identical. For the p telomeres of the acrocentric chromosomes 13, 14, 15, 21 and 22, it is not possible to design reliable probes. For these chromosomes, the P069 probemix only contains a probe for the q telomeric region. The P036 and P070 probemixes contain a second probe for these q arms, which target a DNA sequence close to the centromere. We recommend using SALSA MLPA kit P036 Human Telomere-3 and SALSA MLPA kit P070 Human Telomere-5 for primary screening of the subtelomeres. Results obtained with these kits can be confirmed and further characterized with one of the specific follow up kit for telomere analysis, such as this P208-C1 Human Telomere-6 probemix. |
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