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[求助]
分离方面翻译---汉译英
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| 当pH偏离马铃薯蛋白的等电点时，即pI=7，蛋白质本身所带电荷增加，分子之间的静电斥力增加，使得单位面积气液界面上所吸附的蛋白质的量减少，气泡间的排斥作用增强，气泡间隙增大，液膜厚度增加。而且间隙液中的带电分子或离子与吸附在气液界面上的蛋白质的静电作用增强，使得液膜中液体不容易排到Plateau边界层内，导致泡沫的持液量增加，所以富集比降低而回收率增加。当pH在马铃薯蛋白的等电点时，蛋白质分子所带静电荷为零，分子间排斥力减小，单位面积气液界面上所吸附的蛋白质的量增加。与此同时，气泡间的排斥力减小，液膜内的液体能快速的流到Plateau边界层内，进而在重力和离心力的作用下快速的排到螺旋内构件的壁上，所以泡沫的持液量较低，富集比提高，而收集到的消泡液体积减少，导致蛋白质的回收率降低 |
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【答案】应助回帖
ljzm(金币+90, 翻译EPI+1): 2011-10-28 09:35:06
| When pH is deviated from the isoelectric point of potato protein, which is that pI equals to 7, the charge on protein itself and static electricity repulsion increase. So it resulted in decrease in amount of attached protein on unit area of gas-liquid boundary, increase in repulsion force between bubbles, increase in interspace of bubbles, and increase in thickness of liquid membrane. Also the static electric repulsion between charged molecules as well as ions in interspace liquid and protein attached on gas-liquid boundary, which makes liquid in membrane hard to drain into Plateau boundary layer and liquid hold of foam increases, so the enrichment ratio decreases and recycling ratio increases. On the other hand, when pH is on the isoelectric point of potato protein, the static charge on protein is zero and repulsion between molecules decreases and attached amount of protein on gas-liquid boundary increases. Meanwhile, the repulsion between bubbles decreases and liquid in membrane can drain into Plateau boundary layer quickly. Moreover, the liquid could drain quickly to wall of inner spinal structure under force of gravity and centrifuge. Therefore, the liquid hold of foam is lower while enrichment ratio increases, and volume of collected bubble-free liquid decreases and recycling ratio of protein decreases. |
2楼2011-10-27 12:56:43