| 查看: 279 | 回复: 0 | ||
[求助]
帮翻译一段文章 有关于猪的KIT基因的
|
|
Genetic heterogeneity and selection signature at the KIT gene in pigs showing different coat colours and patterns（题目） Sequencing of the KIT gene The complete coding region of the KIT gene was sequenced in 35 pigs of different breeds: 10 Nero Siciliano pigs, four with completely black coat colour, four with white spots but without duplication of the KIT gene, two with white spots but with the duplication of the KIT gene; three grey animals (one was homozygous for intron 18 deletion and two were heterozygous at this polymorphic site); six Cinta Senese; four Hampshire; four Duroc, two Italian Large White, one carrying the splice mutation and one not carrying this mutation; two Pietrain, one positive and one negative at the duplication test; two Meishan; two wild boars. PCR primers were designed with PRIMER3 (https://fokker.wi.mit.edu/ primer3/input.htm) using pig genomic sequence AC141857 (clone RP44-473N18) containing the 21 KIT exons. BLASTN (bl2seq) analysis (https://blast.ncbi.nlm.nih.gov/Blast.cgi) with cDNA sequences of the porcine KIT gene was used to identify exon borders in the genomic sequence. PCR primers were placed in the intronic region except, for the last exon, which contains a long 3 untranslated region (UTR). PCR primers and amplification conditions are reported in Table S1. Sequences were obtained as described in Table S1. Sequences were assembled and analysed and polymorphisms were detected using CodonCode Aligner (https:// www.codoncode.com/aligner), with the AC141857 sequence included as a reference. All sequence chromatograms were visually inspected. 手机编辑，希望有高手帮翻译一下，先谢谢了！ [ Last edited by yzwan1 on 2011-10-28 at 19:07 ] |
回复此楼» 猜你喜欢
实名举报：华南理工大学物理与光电学院副院长李志远婚内两次出轨女学生（博士）
已经有16人回复
-
上海工程技术大学激光智能制造课题组｜2027级博士研究生招生公告
已经有11人回复
-
上海工程技术大学激光智能制造课题组招收博士研究生
已经有10人回复
-
申博发邮件
已经有11人回复
-
现代”学阀”该如何界定
已经有7人回复
-
各位大神，目前国内有哪些比较好用的逆合成软件？
已经有12人回复
-
我的奶奶
已经有3人回复
-
国社科系统bug了，是不是要放榜了？
已经有9人回复