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binxman木虫 (著名写手)
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[求助]
60金币求助英译中，禁止机器翻译，关于干细胞的，谢谢！
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Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells Transcription factors (TFs) and their specific interactions with targets are crucial for specifying gene-expression programs. To gain insights into the transcriptional regulatory networks in embryonic stem (ES) cells, we use chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing (ChIP-seq) to map the locations of 13 sequence-specific TFs (Nanog, Oct4, STAT3, Smad1, Sox2, Zfx, c-Myc, n-Myc, Klf4, Esrrb, Tcfcp2l1, E2f1, and CTCF) and 2 transcription regulators (p300 and Suz12). These factors are known to play different roles in ES-cell biology as components of the LIF and BMP signaling pathways, self-renewal regulators, and key reprogramming factors. Our study provides insights into the integration of the signaling pathways into the ES-cell-specific transcription circuitries. Intriguingly, we find specific genomic regions extensively targeted by different TFs. Collectively, the comprehensive mapping of TF-binding sites identifies important features of the transcriptional regulatory networks that define ES-cell identity. |
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fuaixiang
金虫 (小有名气)
- 翻译EPI: 23
- 应助: 0 (幼儿园)
- 金币: 1024.8
- 帖子: 56
- 在线: 27.6小时
- 虫号: 1009478
- 注册: 2010-05-01
- 专业: 人体解剖与组织胚胎学
【答案】应助回帖
binxman(金币+60, 翻译EPI+1): 谢谢！太感谢了！能交个朋友吗？584840913 2011-10-20 22:53:12
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胚胎干细胞的外部信号通路与内部转录网络的整合 转录因子(TFs)及其与靶基因之间的特异性相互作用是说明基因表达程序的关键。为了深入理解ES细胞的转录调节网络，我们用染色质免疫沉淀法和超高通量DNA测序(ChIP-seq)来呈现13种TFs (Nanog, Oct4, STAT3, Smad1, Sox2, Zfx, c-Myc, n-Myc, Klf4, Esrrb, Tcfcp2l1, E2f1, and CTCF)和2种转录调节因子(p300 and Suz12)的位置。已知这些因子在胚胎干细胞生物学中起不同的作用，包括LIF和BMP信号通路的组成部分、自我更新因子和关键的重新编程因子。我们的研究提供了如下理解：将信号通路整合到ES细胞特异性转录电路中去。有趣的是，我们发现不同的TFs广泛地针对特定基因组区域。总的来说，TF结合位点的全面呈现确定了决定ES细胞种类的转录调节网络的重要特点。 |
2楼2011-10-20 22:08:58
