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binxman木虫 (著名写手)
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[求助]
80金币求助英译中，禁止机器翻译，谢谢！
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A HEX-1 crystal lattice required for Woronin body function in Neurospora crassa. The Woronin body is a dense-core vesicle specific to filamentous ascomycetes (Euascomycetes), where it functions to seal the septal pore in response to cellular damage. The HEX-1 protein self-assembles to form this solid core of the vesicle. Here, we solve the crystal structure of HEX-1 at 1.8 A, which provides the structural basis of its self-assembly. The structure reveals the existence of three intermolecular interfaces that promote the formation of a three-dimensional protein lattice. Consistent with these data, self-assembly is disrupted by mutations in intermolecular contact residues and expression of an assembly-defective HEX-1 mutant results in the production of aberrantWoronin bodies, which possess a soluble noncrystalline core. This mutant also fails to complement a hex-1 deletion in Neurospora crassa, demonstrating that the HEX-1protein lattice is required for Woronin body function. Although both the sequence and the tertiary structure of HEX-1 are similar to those of eukaryotic initiation factor 5A (eIF-5A), the amino acids required for HEX-1 self-assembly and peroxisomal targeting are absent in eIF-5A. Thus, we propose that a new function has evolved following duplication of an ancestral eIF-5A gene and that this may define an important step in fungal evolution. |
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fuaixiang
金虫 (小有名气)
- 翻译EPI: 23
- 应助: 0 (幼儿园)
- 金币: 1024.8
- 帖子: 56
- 在线: 27.6小时
- 虫号: 1009478
- 注册: 2010-05-01
- 专业: 人体解剖与组织胚胎学
【答案】应助回帖
binxman(金币+80, 翻译EPI+1): 谢谢！太感谢了！救命恩人啊 2011-10-21 23:09:45
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粗糙链孢菌中乌头类生物碱功能所需的HEX-1晶格 乌头类生物碱是一种有致密核的囊泡，对丝状子囊菌（真子囊菌纲）有特效，其功能是封闭中隔孔以应对细胞损伤。HEX-1蛋白自组装而形成囊泡的实心核。在这里，我们在1.8 A处解开HEX-1的晶体结构，1.8 A提供了其自组装的结构基础。这种结构显示，有三个分子间接口促进三维蛋白晶格的形成。与这些数据一致的是，分子间接触残基的突变干扰了自组装，自组装缺陷HEX-1突变体的表达导致异常乌头类生物碱（具有水溶性非晶核）的产生。这种突变体也不能补足粗糙链孢菌中的hex-1缺失，证明HEX-1蛋白晶格是乌头类生物碱功能所必需的。尽管HEX-1的序列和三级结构都与真核起始因子5A（eIF-5A）类似，但是eIF-5A中缺少HEX-1自组装和过氧化物酶体定位所需的氨基酸。因此，我们认为，原始eIF-5A基因复制后形成了一种新功能，这可能决定了真菌进化中的一个重要步骤。 |
2楼2011-10-21 21:33:56
