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binxman木虫 (著名写手)
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[求助]
80金币求助英译中，禁止机器翻译，关于干细胞的，谢谢！
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Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells. The histone H3 Lys 9 (H3K9) methyltransferase Eset is an epigenetic regulator critical for the development of the inner cell mass (ICM). Although ICM-derived embryonic stem (ES) cells are normally unable to contribute to the trophectoderm (TE) in blastocysts, we find that depletion of Eset by shRNAs leads to differentiation with the formation of trophoblast-like cells and induction of trophoblast-associated gene expression. Using chromatin immmunoprecipitation (ChIP) and sequencing (ChIP-seq) analyses, we identified Eset target genes with Eset-dependent H3K9 trimethylation. We confirmed that genes that are preferentially expressed in the TE (Tcfap2a and Cdx2) are bound and repressed by Eset. Single-cell PCR analysis shows that the expression of Cdx2 and Tcfap2a is also induced in Eset-depleted morula cells. Importantly, Eset-depleted cells can incorporate into the TE of a blastocyst and, subsequently, placental tissues. Coimmunoprecipitation and ChIP assays further demonstrate that Eset interacts with Oct4, which in turn recruits Eset to silence these trophoblast-associated genes. Our results suggest that Eset restricts the extraembryonic trophoblast lineage potential of pluripotent cells and links an epigenetic regulator to key cell fate decision through a pluripotency factor. |
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fuaixiang
金虫 (小有名气)
- 翻译EPI: 23
- 应助: 0 (幼儿园)
- 金币: 1024.8
- 帖子: 56
- 在线: 27.6小时
- 虫号: 1009478
- 注册: 2010-05-01
- 专业: 人体解剖与组织胚胎学
【答案】应助回帖
★ ★
爱与雨下(金币+2): 2011-10-22 09:56:02
binxman(金币+80, 翻译EPI+1): 谢谢！太给力啦 2011-10-22 19:13:54
爱与雨下(金币+2): 2011-10-22 09:56:02
binxman(金币+80, 翻译EPI+1): 谢谢！太给力啦 2011-10-22 19:13:54
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胚胎干细胞中的Eset联合Oct4限制胚外滋养层细胞谱系的潜能 组蛋白H3 Lys 9 (H3K9) 甲基转移酶Eset是内细胞群(ICM)发育所必需的一种渐成说的调节因子。虽然ICM衍生的胚胎干(ES)细胞正常情况下不能促进囊胚的滋养外胚层(TE)的发育，但是我们发现，shRNAs消耗Eset后导致分化，形成滋养层样细胞并诱导滋养层相关基因的表达。我们使用染色质免疫沉淀(ChIP)及测序(ChIP-seq)分析，通过Eset依赖H3K9甲基化识别了Eset靶基因。我们确认在TE (Tcfap2a 和Cdx2)中优先表达的基因受Eset的约束。单细胞PCR分析显示在Eset减少的桑葚细胞中还诱导了Cdx2 和Tcfap2a的表达。值得一提的是，Eset减少的细胞可进入胚泡TE，随后进入胚盘组织。联合免疫沉淀及ChIP法进一步证明，Eset 与Oct4相互作用，反过来吸收Eset，从而抑制滋养层相关基因。我们的实验结果提示Eset限制了多能干细胞的胚外滋养层细胞谱系的潜能，通过多能性因子将渐成说的调节因子与重要细胞结局的确定联系起来。 |
2楼2011-10-22 01:12:41