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binxman木虫 (著名写手)
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[求助]
80金币求助英译中，禁止机器翻译，谢谢！
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A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. Geminin is a cellular protein that associates with Cdt1 and inhibits Mcm2-7 loading during S phase. It prevents multiple cycles of replication per cell cycle and prevents episome replication. It also directly inhibits the HoxA11 transcription factor. Here we report that geminin forms a parallel coiled-coil homodimer with atypical residues in the dimer interface. Point mutations that disrupt the dimerization abolish interaction with Cdt1 and inhibition of replication. An array of glutamic acid residues on the coiled-coildomain surface interacts with positive charges in the middle of Cdt1. An adjoining region interacts independently with the N-terminal 100 residues of Cdt1. Both interactions are essential for replication inhibition. The negative residues on the coiled-coil domain and a different part of geminin are also required for interaction with HoxA11. Therefore a rigid cylinder with negative surface charges is a critical component of a bipartite interaction interface between geminin and its cellular targets. |
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fuaixiang
金虫 (小有名气)
- 翻译EPI: 23
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- 注册: 2010-05-01
- 专业: 人体解剖与组织胚胎学
【答案】应助回帖
binxman(金币+80, 翻译EPI+1): 谢谢！太给力啦 2011-10-22 00:40:54
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孪蛋白的二聚卷曲螺旋域和毗连部位通过与Cdt1上的两个位点相互作用而抑制复制 孪蛋白是一种与Cdt1有关的细胞蛋白，在S阶段抑制Mcm2-7装载。它通过细胞周期阻止多周期复制，并阻止游离基因的复制。它还直接抑制HoxA11转录因子。这里，我们作出如下报道：孪蛋白形成平行卷曲螺旋同型二聚体，在二聚体交界面有非典型残基。破坏了二聚体的点突变消除了与Cdt1的相互作用及复制的抑制。卷曲螺旋域表面的一列谷氨酸残基与Cdt1中间的正电荷相互作用。毗连区域独立地与Cdt1的N端100个残基相互作用。两种相互作用都是抑制复制所必需的。卷曲螺旋域的阴性残基和孪蛋白的另一不同部位也是与HoxA11相互作用所必需的。因此，带阴性表面电荷的刚性圆柱体是孪蛋白及其靶细胞之间的两个相互作用界面关键的组成部分。 |
2楼2011-10-22 00:25:44
