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binxman木虫 (著名写手)
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[求助]
100金币求助英译中，禁止机器翻译，关于干细胞的，谢谢！
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Tbx3 improves the germ-line competency of induced pluripotent stem cells. Induced pluripotent stem (iPS) cells can be obtained by the introduction of defined factors into somatic cells. The combination of Oct4 (also known as Pou5f1), Sox2 and Klf4 (which we term OSK) constitutes the minimal requirement for generating iPS cells from mouse embryonic fibroblasts. These cells are thought to resemble embryonic stem cells (ESCs) on the basis of global gene expression analyses; however, few studies have tested the ability and efficiency of iPS cells to contribute to chimaerism, colonization of germ tissues, and most importantly, germ-line transmission and live birth from iPS cells produced by tetraploid complementation. Using genomic analyses of ESC genes that have roles in pluripotency and fusion-mediated somatic cell reprogramming, here we show that the transcription factor Tbx3 significantly improves the quality of iPS cells. iPS cells generated with OSK and Tbx3 (OSKT) are superior in both germ-cell contribution to the gonads and germ-line transmission frequency. However, global gene expression profiling could not distinguish between OSK and OSKT iPS cells. Genome-wide chromatin immunoprecipitation sequencing analysis of Tbx3-binding sites in ESCs suggests that Tbx3 regulates pluripotency-associated and reprogramming factors, in addition to sharing many common downstream regulatory targets with Oct4, Sox2, Nanog and Smad1. This study underscores the intrinsic qualitative differences between iPS cells generated by different methods, and highlights the need to rigorously characterize iPS cells beyond in vitro studies. |
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fuaixiang
金虫 (小有名气)
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- 专业: 人体解剖与组织胚胎学
【答案】应助回帖
binxman(金币+100, 翻译EPI+1): 谢谢！不好意思啊，要你搞到那么晚 2011-10-22 00:41:19
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Tbx3促进诱导多能干细胞的生殖细胞系能力 诱导多能干细胞(iPS)可通过将确定的因子引入体细胞的方法获得。从小鼠胚胎成纤维细胞产生iPS细胞至少需要Oct4 (亦称之为Pou5f1)、Sox2 和 Klf4 (我们称之为OSK)的结合。有人根据综合基因表达分析，认为这些细胞组装了胚胎干细胞(ESCs)；但是，几乎没有研究测试iPS细胞在生殖组织的嵌合现象和定植尤其是生殖细胞系传递以及通过四倍体互补产生的iPS细胞生成活体生殖细胞系中的能力和效果。我们对在多能化和融合介导体细胞重新编程中起作用的ESC基因进行基因组分析，显示转录因子Tbx3显著提高了iPS细胞的性能。iPS细胞生成OSK 和Tbx3 (OSKT)，在促进性腺产生生殖细胞及生殖细胞系传递频率中都占有优势。但是，综合基因表达不能区分OSK 和OSKT iPS 细胞。对ESC的Tbx3结合位点进行基因组范围的染色质免疫沉淀测序分析提示，除了共享许多共同的下游调节靶基因包括Oct4, Sox2, Nanog 和 Smad1，Tbx3调节多能相关因子和重新编程因子。本研究强调了通过不同方法生成的iPS 细胞之间的内在性质的差异，强调了有必要在体外试验范围之外精确地描述iPS细胞的特性。 |
2楼2011-10-22 00:24:14
binxman
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3楼2011-10-23 00:44:13