| ²é¿´: 972 | »Ø¸´: 2 | ||||
| µ±Ç°Ö÷ÌâÒѾ´æµµ¡£ | ||||
rosebud123Ìú³æ (³õÈëÎÄ̳)
|
[½»Á÷]
Àë×Ó½»»»É«Æ×·¨
|
|||
°ïæ¶¥¶¥Ñ½![]() ![]() ![]() ![]() Sober ºÍ PetersonÓÚ1956ÄêÊ״ν«Àë×Ó½»»»»ùÍŽáºÏµ½ÏËÎ¬ËØÉÏ£¬ÖƳÉÁËÀë×Ó½»»»ÏËÎ¬ËØ£¬³É¹¦µØÓ¦ÓÃÓÚµ°°×ÖʵķÖÀë¡£´Ó´ËʹÉúÎï´ó·Ö×ӵķּ¶·ÖÀë·½·¨È¡µÃÁËѸËٵķ¢Õ¹¡£Àë×Ó½»»»»ùÍŲ»µ«¿É½áºÏµ½ÏËάÉÏ£¬ »¹¿É½áºÏµ½½»ÁªÆÏ¾ÛÌÇ£¨S-ephadex£©ºÍÇíÖ¬ÌÇÄý½º£¨Sepharose£©ÉÏ¡£ ½üÄêÀ´Àë×Ó½»»»É«Æ×¼¼ÊõÒѾ¹ã·ºÓ¦ÓÃÓÚµ°°×ÖÊ¡¢Ã¸¡¢ºËËá¡¢ëÄ¡¢¹ÑºËÜÕËá¡¢²¡¶¾¡¢ÊɾúÌåºÍ¶àÌǵķÖÀëºÍ´¿»¯¡£ËüÃǵÄÓŵãÊÇ:¢Å¾ßÓпª·ÅÐÔÖ§³Ö¹Ç¼Ü£¬´ó·Ö×Ó¿ÉÒÔ×ÔÓɽøÈëºÍѸËÙÀ©É¢£¬¹ÊÎü¸½ÈÝÁ¿´ó¡£¢Æ¾ßÓÐÇ×Ë®ÐÔ£¬¶Ô´ó·Ö×ÓµÄÎü¸½²»´óÀι̣¬ÓÃκÍÌõ¼þʹ¿ÉÒÔÏ´ÍÑ£¬²»ÖÂÒýÆðµ°°×ÖʱäÐÔ»òøµÄʧ»î¡£¢Ç¶à¿×ÐÔ£¬±íÃæ»ý´ó¡¢½»»»ÈÝÁ¿´ó£¬»ØÊÕÂʸߣ¬¿ÉÓÃÓÚ·ÖÀëºÍÖÆ±¸¡£ Ò»¡¢»ù±¾ÀíÂÛ ¡¡¡¡Àë×Ó½»»»¼Áͨ³£ÊÇÒ»ÖÖ²»ÈÜÐԸ߷Ö×Ó»¯ºÏÎÈçÊ÷Ö¬£¬ÏËÎ¬ËØ£¬ÆÏ¾ÛÌÇ£¬´¼Ö¬Ìǵȣ¬ËüµÄ·Ö×ÓÖк¬ÓпɽâÀëµÄ»ùÍÅ£¬ÕâЩ»ùÒòÔÚË®ÈÜÒºÖÐÄÜÓëÈÜÒºÖÐµÄÆäËüÑôÀë×Ó»òÒõÀë×ÓÆð½»»»×÷Óá£ËäÈ»½»»»·´Ó¦¶¼ÊÇÆ½ºâ·´Ó¦£¬µ«ÔÚ²ãÎöÖùÉϽøÐÐʱ£¬ÓÉÓÚÁ¬ÐøÌí¼ÓеĽ»»»ÈÜÒº£¬Æ½ºâ²»¶Ï°´Õý·½Ïò½øÐУ¬Ö±ÖÁÍêÈ«¡£Òò´Ë¿ÉÒÔ°ÑÀë×Ó½»»»¼ÁÉϵÄÔ×ÓÀë×ÓÈ«²¿Ï´ÍÑÏÂÀ´£¬Í¬Àí£¬µ±Ò»¶¨Á¿µÄÈÜҺͨ¹ý½»»»Öùʱ£¬ÓÉÓÚÈÜÒºÖеÄÀë×Ó²»¶Ï±»½»»»¶ø²¨¶ÈÖð¼õÉÙ£¬Òò´ËÒ²¿ÉÒÔÈ«²¿±»½»»»²¢Îü¸½ÔÚÊ÷Ö¬ÉÏ¡£Èç¹ûÓÐÁ½ÖÖÒÔÉϵijɷֱ»½»»»Îü×ÅÔÚÀë×Ó½»»»¼ÁÉÏ£¬ÓÃÏ´ÍÑҺϴÍÑʱ£¬ÔÚ±»Ï´ÍѵÄÄÜÁ¦Ôò¾ö¶¨ÓÚ¸÷×ÔÏ´·´Ó¦µÄƽºâ³£Êý¡£µ°°×ÖʵÄÀë×Ó½»»»¹ý³ÌÓÐÁ½¸ö½×¶Î©¤©¤Îü¸½ºÍ½âÎü¸½¡£Îü¸½ÔÚÀë×Ó½»»»¼ÁÉϵĵ°°×ÖÊ¿ÉÒÔͨ¹ý¸Ä±äpHʹÎü¸½µÄµ°°×ÖÊʧȥµçºÉ¶ø´ïµ½½âÀ뵫¸ü¶àµÄÊÇͨ¹ýÔö¼ÓÀë×ÓÇ¿¶È£¬Ê¹¼ÓÈëµÄÀë×ÓÓëµ°°×ÖʾºÕùÀë×Ó½»»»¼ÁÉϵĵçºÉλÖã¬Ê¹Îü¸½µÄµ°°×ÖÊÓëÀë×Ó½»»»¼Á½â¿ª¡£²»Í¬µ°°×ÖÊÓëÀë×Ó½»»»¼ÁÖ®¼äÐγɵç¼üÊýÄ¿²»Í¬£¬¼´Ç׺ÍÁ¦´óСÓвîÒì £¬Òò´ËֻҪѡÔñÊʵ±µÄÏ´ÍÑÌõ¼þ±ã¿É½«»ìºÏÎïÖеÄ×é·ÖÖð¸öÏ´ÍÑÏÂÀ´£¬´ïµ½·ÖÀë´¿»¯µÄÄ¿µÄ¡£ ¶þ¡¢Àë×Ó½»»»µÄ·ÖÀ༰³£¼ûÖÖÀà £¨Ò»£©·ÖÀà Àë×Ó½»»»¼Á·ÖΪÁ½´óÀ࣬¼´ÑôÀë×Ó½»»»¼ÁºÍÒõÀë×Ó½»»»¼Á¡£¸÷Àཻ»»¼Á¸ù¾ÝÆä½âÀëÐÔ´óС£¬»¹¿É·ÖΪǿ¡¢ÈõÁ½ÖÖ£¬¼´ Ç¿Ëá¼Á¡¡ÑôÀë×Ó½»»»¼Á ¡¡ÈõËá¼Á¡¡Ç¿¼ïÐÍ¡¡ÒõÀë×Ó½»»»¼Á¡¡Èõ¼ïÐÍ ¡£ 1.ÑôÀë×Ó½»»»¼Á ¡¡¡¡ÑôÀë×Ó½»»»¼ÁÖеĿɽâÀë»ùÒòÊÇ»ÇËᣨ-SO3H£©¡¢Á×Ëᣨ-PO3H2£©¡¢ ôÈËᣨCOOH£©ºÍ·ÓôÇ»ù£¨-OH£©µÈËáÐÔ»ù¡£ ijЩ½»»»¼ÁÔÚ½»»»Ê±·´Ó¦ÈçÏ£º Ç¿ËáÐÔ£ºR-SO3 -H+ £« Na+ R-SO3- Na+H+ ÈõËáÐÔ£ºR-COOH£«Na+ R-COONa £«H+ ¹ú²úÊ÷Ö¬ÖÐÇ¿Ëá1×7£¨ÉϺ£Ê÷Ö¬££732£©ºÍ¹úÍâ²úÆ·Dowex 50¡¢Zerolit 225µÈ¶¼ÓÚÇ¿ËáÐÍÀë×Ó½»»»¼Á¡£ 2.ÒõÀë×Ó½»»»¼Á ¡¡¡¡ÒõÀë×Ó½»»»¼ÁÖеĿɽâÀë»ùÒòÊDz®°·¡¢£¨-NH2£©¡¢ÖÙ°·£¨-NHCH3£©¡¢Êå°·[N-£¨CH3£©2]ºÍ¼¾°·[-N£¨CH3£©2]µÈ¼ïÐÔ»ùÍÅ¡£Ä³Ð©½»»»·´Ó¦ÈçÏ£º Ç¿¼ïÐÔ£ºR-N+£¨CH3£©2 H·OH- £«Cl R-N+£¨CH3£©2 Cl£«OH- Èõ¼ïÐÔ£ºR-N+£¨CH3£©2 H·OH- £«Cl R-N+£¨CH3£©2 HCl£«OH- Ç¿¼ïÐÔ££201ºÅ¹ú²úÊ÷Ö¬ºÍ¹úÍâDowex1¡¢Dowex2¡¢ZerolitFFµÈ¶¼ÊôÓÚÇ¿¼ïÐÍÒõÀë×Ó½»»»¼Á¡£ £¨¶þ£©ÖÖÀà 1.ÏËÎ¬ËØÀë×Ó½»»»¼Á£ºÑôÀë×Ó½»»»¼ÁÓÐôǼ׻ùÏËÎ¬ËØ£¨CM-ÏËÎ¬ËØ£©£¬ ÒõÀë×Ó½»»»¼ÁÓÐÂÈ´úÈýÒÒ°·ÏËάɴ£¨DESE-ÏËÎ¬ËØ£©¡£ 2.½»ÁªÆÏ¾ÛÌÇÀë×Ó½»»»¼Á£ºÊǽ«½»»»»ùÒòÁ¬½Óµ½½»ÁªÆÏ¾ÛÌÇÉÏÖÆ³ÉµÄÒ»Àཻ»»¼Á£¬Òò¶ø¼È¾ßÓÐÀë×Ó½»»»×÷Óã¬ÓÖ¾ßÓзÖ×ÓɸЧӦ£¬ÊÇÒ»Àà¹ã·ºÓ¦ÓõÄÉ«Æ×·ÖÀëÎïÖÊ¡£³£ÓõÄSephadexÀë×Ó½»»»¼ÁÒ²ÓÐÒõÀë×ÓºÍÑôÀë×Ó½»»»¼ÁÁ½Àà¡£ÒõÀë×Ó½»»»¼ÁÓÐDEAE-Sephadex A-25£¬A-50ºÍQAE- Sephadex A25 £¬ A50 £» ÑôÀë×Ó½»»»¼ÁÓÐCM-Sephaetx C-50£¬C-50ºÍSephadex C-25£¬C-50¡£ÒõÀë×Ó½»»»¼ÁÓÃÓ¢ÎÄ×ÖÍ·A£¬ÑôÀë×Ó½»»»¼ÁµÄÓ¢ÎÄ×ÖÍ·ÊÇC¡£Ó¢ÎÄ×ÖºóÃæµÄÊý×Ö±íʾSephadexÐͺš£ 3.ÇíÖ¬ÌÇÀë×ÓÀë½»»»¼Á£ºÊǽ«DESE-»òCM-»ùÍŸ½×ÅÔÚSepharose CL-6B ÉÏÐγɣ¬DEAE-Sephades£¨ÒõÀë×Ó£©ºÍCM-Sepharose£¨ÑôÀë×Ó£©£¬¾ßÓÐÓ²¶È´ó£¬ ÐÔÖÊÎȶ¨£¬Äý½ººóµÄÁ÷Ëٺ㬷ÖÀëÄÜÁ¦Ç¿µÈÓŵ㡣 Èý¡¢ÊµÑé²Ù×÷ £¨Ò»£©½»»»¼ÁµÄ´¦Àí£¬ÔÙÉúÓëתÐÍ ¡¡¡¡Ð³ö³§µÄÊ÷Ö¬ÊǸÉÊ÷Ö¬£¬ÒªÓÃË®½þ͸ʹ֮³ä·ÖÎüË®ÅòÕÍ¡£ÒòÆäº¬ÓÐÕþ¼¨Ò»Ð©ÔÓÖÊ£¬ËùÒªÒªÓÃË®¡¢Ëá¡¢¼ïÏ´µÓ¡£Ò»°ãÊÖÐøÈçÏ£ºÐ³ö³§¸ÉÊ÷Ö¬ÓÃË®½þÅÝ2 Сʱºó¼õ³éÑ¹È¥ÆøÅÝ£¬Çãȥˮ£¬ÔÙÓôóÁ¿ÎÞÀë×ÓˮϴÖÁ³ÎÇ壬ȥˮºó¼Ó4±¶Á¿2N HCl½Á¶¶4Сʱ£¬³ýÈ¥ËáÒº£¬Ë®Ï´µ½ÖÐÐÔ£¬ÔÙ¼Ó4±¶Á¿2N NaOH½Á¶¶4Сʱ£¬³ý¼ïÒº£¬ ˮϴµ½ÖÐÐÔ±¸Ó᣽«Ê÷Ö¬´øÉÏËùÏ£ÍûµÄijÖÖÀë×ӵIJÙ×÷³ÆÎª×ªÐÍ¡£ÈçÏ£ÍûÑôÊ÷Ö¬´øNa+£¬ÔòÓÃ4±¶Á¿NaOH½Á°è½þÅÝ2СʱÒÔÉÏ£»ÈçÏ£ÍûÊ÷Ö¬´øH+£¬¿ÉÓÃHCl´¦Àí¡£ÒõÊ÷֬תÐÍҲͬÑù£¬ÈôÏ£Íû´øCl-ÔòÓÃHCl£¬Ï£Íû´øOH-ÔòÓÃNaOH¡£ÓùýµÄÊ÷֬ʹÆä»Ö¸´Ô×´µÄ·½·¨³ÆÎªÔÙÉú¡£²¢·Çÿ´ÎÔÙÒ»¶¼ÓÃËá¡¢¼ïҺϴµÓ£¬ÍùÍùֻҪתÐÍ´¦Àí¾ÍÐÐÁË¡£ £¨¶þ£©ÖùÉϲÙ×÷ ¢Å½»»»¼Á×°Öù×î¼òµ¥µÄ½»»»²ãÎöÖù¿ÉÓüïʽµÎ¶¨¹Ü´úÌæ¡£´¦Àí¹ýµÄÊ÷Ö¬·ÅÈëÉÕ±£¬¼ÓÉÙÁ¿Ë®±ß½Á°è±ßµ¹Èë±£³Ö´¹Ö±µÄ²ãÎö¹ÜÖУ¬Ê¹Ê÷Ö¬»ºÂý³Á½µ¡£½»»»¼ÁÔÚÖùÄÚ±ØÐë·Ö²¼¾ùÔÈ¡£ ¢ÆÉÏÑùÏò²ãÎöÖùÄÚÇãÈëÑùÆ·Òº ¢ÇÏ´ÍÑÓëÊÕ¼¯ ¡¡¡¡²»Í¬ÑùÆ·Ñ¡ÓõÄÏ´ÍÑÒº²»Í¬¡£ÔÔòÊÇÓÃÒ»ÖÖ±ÈÎü×ÅÎïÖʸü»îÆÃµÄÀë×Ó£¬°ÑÎü×ÅÎï½»»»³öÀ´¡£ÓÉÓÚ±»Îü×ŵÄÎïÖÊÍùÍù²»ÊÇÎÒÃÇËùÒªÇóµÄµ¥Ò»ÎïÖÊ£¬Òò´Ë³ýÁËÕýÈ·Ñ¡ÔñÏ´ÍÑÒºÍ⻹²É¿ØÖÆÁ÷Ëٺͷֲ¼ÊÕ¼¯µÄ·½·¨À´»ñµÃËùÐèµÄµ¥Ò»ÎïÖÊ¡£ [ Last edited by mxg330 on 2007-6-30 at 02:33 ] |
» ÊÕ¼±¾ÌûµÄÌÔÌûר¼ÍƼö
It's useful |
» ²ÂÄãϲ»¶
Çóµ÷¼Á
ÒѾÓÐ3È˻ظ´
265Çóµ÷¼Á
ÒѾÓÐ4È˻ظ´
085700×ÊÔ´Óë»·¾³308Çóµ÷¼Á
ÒѾÓÐ6È˻ظ´
Ò»Ö¾Ô¸¼ªÁÖ´óѧ²ÄÁÏѧ˶321Çóµ÷¼Á
ÒѾÓÐ12È˻ظ´
286·ÖÈ˹¤ÖÇÄÜרҵÇëÇóµ÷¼ÁÔ¸Òâ¿ç¿¼£¡
ÒѾÓÐ3È˻ظ´
329Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
ÉêÇë»Ø¸åÑÓÆÚÒ»¸öÔ£¬±à¼Í¬ÒâÁË¡£µ«ÏµÍ³ÉϵÄʱ¼äû±ä£¬¸ø±à¼ÓÖдÓʼþÁË£¬Ã»»Ø¸´
ÒѾÓÐ4È˻ظ´
²ÄÁÏѧ˶318Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
Ò»Ö¾Ô¸Öйúº£Ñó´óѧ£¬ÉúÎïѧ£¬301·Ö£¬Çóµ÷¼Á
ÒѾÓÐ6È˻ظ´
081700»¯¹¤Ñ§Ë¶µ÷¼Á
ÒѾÓÐ3È˻ظ´
» ±¾Ö÷ÌâÏà¹ØÉ̼ÒÍÆ¼ö: (ÎÒÒ²ÒªÔÚÕâÀïÍÆ¹ã)
|
2Â¥2006-12-24 10:56:03
chywang2006
Òø³æ (ÕýʽдÊÖ)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ½ð±Ò: 740.1
- Ìû×Ó: 391
- ÔÚÏß: 12.8Сʱ
- ³æºÅ: 316668
- ×¢²á: 2007-03-03
- ÐÔ±ð: MM
- רҵ: ·ÖÎöÒÇÆ÷ÓëÊÔ¼Á
3Â¥2007-03-05 21:54:37

















»Ø¸´´ËÂ¥

10