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Bacterial isolates with the ability to tolerate salinity and plant growth-promoting features were isolated from the saline areas of Gujarat, India, that is, Bhavnagar and Khambat. A total of 176 strains of rhizobacteria were isolated out of which 62 bacterial strains were able to tolerate 1 M NaCl. These were then further studied for their potential plant growth-promoting rhizobacteria characteristics like phosphate solubilization, siderophore production, and IAA production. Twenty-eight isolates of the 62 strains showed good tricalcium phosphate solubilization in solid medium in the range of 9–22 mm and 15 isolates showed good phosphate solubilization in liquid medium in the range of 9–45 lg/ml. Siderophore production was checked in all 15 isolates, and 13 were screened out that produced the hydroxamate type of siderophore in the range of 11–50 mM. Among the 13 isolates, 10 were able to produce indole acetic acid in the range of 10–26 lg/ml after 72 h of incubation. Pot trials were carried out on chickpea under 300 mM NaCl stress using the best five isolates. Plants inoculated with MSC1 or MSC4 isolates showed an increase in the parameters that evaluate plant growth when compared to uninoculated controls. Strains MSC1 and MSC4 were identified as Pseudomonas putida and Pseudomonas pseudoalcaligens, respectively, according to sequence analysis of the 16S rRNA gene. |
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fanliang1389
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爱与雨下(金币+1): ！！ 2011-09-26 21:52:24
ydd1982(金币+50, 翻译EPI+1): 虽然觉得不是非常专业，但是能够给我翻译，我已经不胜感激，多谢，金币全赏了，有另外补充的话，我会再追加金币。 2011-09-27 16:27:03
爱与雨下(金币+1): ！！ 2011-09-26 21:52:24
ydd1982(金币+50, 翻译EPI+1): 虽然觉得不是非常专业，但是能够给我翻译，我已经不胜感激，多谢，金币全赏了，有另外补充的话，我会再追加金币。 2011-09-27 16:27:03
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在印度的Gujarat的盐渍地区即Bhavnagarand Khambat中分离了一些具有耐盐性且促进植物生长特定的细菌菌株，从中一共分离出了176株根际细菌，其中有62株在1M的NaCl中耐受。 进一步研究这些菌株的可能促进植物生长的根际细菌特性，如磷酸盐溶解作用，含铁细胞和IAA的产量。 这62株菌株中有28株在固体培养基中显示了良好的磷酸三钙溶解性，溶解范围为9-22mm；有15株在液体培养基中显示了良好的磷酸盐溶解性，溶解范围为9–45 lg/ml。 检验了所有这15中菌株的含铁细胞的产量，其中筛选出了13株菌，因其能产生氧肟酸盐型的含铁细胞，产生范围为在11–50 mM。 这13株菌种，培养72小时以后，十种能够产生吲哚乙酸，产生范围为10–26 lg/ml 采用其中最好的5株菌株在300mM的NaCl溶液浓度下对鹰嘴豆进行盆栽实验。 接种MSC1或MSC4菌株的植物的生长参数高于未接种的植物。 根据16S RNA基因序列分析结果得知，菌株MSC1和MSC4分别为恶臭假单胞菌和Pseudomonas pseudoalcaligens（也是假单细胞菌属的一种，没有查出来） |
2楼2011-09-26 20:51:28
爱与雨下: 那是版主额外给的，鼓励奖！！ 2011-09-27 08:47:46
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3楼2011-09-26 21:59:53
