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bamage木虫 (著名写手)
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[求助]
求助一段生物学方面文献的翻译
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求助分子生物学方面的一段文献翻译，谢谢！ Determination of Distance Restraints from PREs PRE-derived distances were determined from methyl-TROSY spectra of SecA by measuring peak intensities before (paramagnetic) and after (diamagnetic) reduction of the nitroxide spin label with ascorbic acid. PRE values were then converted to distances by using a modified Solomon-Bloembergen equation for transverse relaxation, as described previously. Two sets of restraints were incorporated into subsequent structure calculations. Methyl groups strongly affected by the presence of the spin label in the peptide and whose resonances broaden beyond detection in the paramagnetic spectrum, were restrained with only an upper bound distance estimated from the noise of the spectrum plus 4 Å. Methyl groups whose resonances appear in the paramagnetic spectra were restrained as the calculated distance with ± 4 Å upper/lower bounds. [ Last edited by bamage on 2011-9-25 at 12:07 ] |
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【答案】应助回帖
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爱与雨下(金币+1): ！~ 2011-09-26 12:06:23
bamage(金币+3, 翻译EPI+1): 谢谢回帖！ 2011-09-27 18:00:33
bamage(金币+5): 2011-09-27 20:34:23
bamage(金币+12): 2011-09-29 17:04:39
爱与雨下(金币+1): ！~ 2011-09-26 12:06:23
bamage(金币+3, 翻译EPI+1): 谢谢回帖！ 2011-09-27 18:00:33
bamage(金币+5): 2011-09-27 20:34:23
bamage(金币+12): 2011-09-29 17:04:39
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由PREs中检测距离检测限 由SecA中的甲-trosy光谱检测PRE的导出距离，该检测距离是通过检测硝基氧自旋标记物与抗坏血酸在减弱前（顺磁性）和减弱后（逆磁性）来实现的。之后，采用更改过的Solomon-Bloembergen方程，将PRE值转化为距离，采用该方程的目的在于横向松弛，此方法之前已经进行了描述。两组装置结合为之后的结构计算。受到肽中的自旋标记物的强烈影响且其共振态加宽并超出顺次共振谱的检测范围的甲基原子团只受最大距离的限制，该距离由光谱的噪音+4A估算而出。在顺磁光谱中可以看到甲基原子团，其最大/最小检测距离分别为计算值± 4 Å |
2楼2011-09-26 11:47:04
