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[求助]
帮忙看看高血压文献英译中的错误
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Protocol The mice were synchronized to a light–dark schedule of 12–12 hours, with lights on at 06:00 hours. First recordings were started at least 1 week after surgery, when the mice had regained their circadian BP and HR rhythm, and the surgery and anesthesia dependent initial changes were followed by stable values. Baseline measurements were compared with measurements after intraperitoneal injection of the a-2 adrenoceptor blocker, yohimbine (2 mg/kg), and of the a-2 adrenoceptor agonist, clonidine (1, 10, and 50 mg/kg). In addition, the mice were given metoprolol (4 mg/kg), atropine (2 mg/kg), clonidine (50 mg/kg) plus atropine (2 mg/kg), or clonidine (50 mg/kg) plus metoprolol (4 mg/kg) on separate days. All drugs were administered by intraperitoneal injections; 0.9% NaCl solution was used as vehicle. Intraperitoneal injection of adequate volumes of 0.9% NaCl had no sustained cardiovascular effect. 原始试验 老鼠处于12-12明暗日程，6:00开灯。手术至少1周后才开始第一次记录，此时老鼠重新恢复昼夜血压和心率的节奏，手术带来的最初麻醉的依赖初步改变，其次是稳定值。基础测量与注射了a-2受体阻滞剂，育享宾（2mg/kg）和肾上腺受体激动剂，可乐定（1, 10, and 50 mg/kg）后的测量进行比较。另外，老鼠还在不同的白天还给予美托洛尔（4mg/kg），阿托品（2mg/kg），可乐定（50mg/kg）加阿托品（2mg/kg）或可乐定（50mg/kg）加美托洛尔（4mg/kg）。 所有的药物通过腹腔注射；0.9% NaCl溶液作为载体。腹腔注射足够的0.9% NaCl没有持续的心血管影响。 Spectral Analysis and Baroreflex Sensitivity The baroreceptor HR reflex was calculated using the sequence method over the whole 2-hour period.13,17,18 Briefly, the spontaneous BRS was calculated as the slope of the linear regression lines between the systolic blood pressure (SBP) and the subsequent R-R intervals with a delay of 1 heart beat using the sequence technique. Sequences with at least 3 intervals, 0.5 mm Hg BP changes, and 5-ms R-R interval changes were analyzed. BRS was calculated as the mean value of all slopes obtained. HRV was analyzed in the time and frequency domain. As time domain method, we used the square root of the mean squared differences of successive intervals, which reflects short-term variations of HR. The power spectra of SBP and R-R interval time series (segment length 512 beats, resampling with 12 Hz) and the crossspectra10–13,19 were calculated using fast Fourier transformation. The baroreflex gain was determined as mean value of the transfer function in low-frequency (LF) and high-frequency (HF) bands. The frequency bands were adapted for analysis in mice considering the ranges of HR and breathing frequencies (very LF_0.015 to 0.25 Hz, LF_0.25 to 1.0 Hz, HF_1.0 to 6.0 Hz). BRS was considered significant if the coherence in the analyzed frequency band was _0.8. The data analysis was performed by use of PVwave software 频谱分析和压力反射敏感性 在整个2小时期间内，HR反射的压力感受器是使用序列计算法。简单地讲，序列技术计算自发BRS, 采用收缩压(SBP)和随后延迟1个心脏跳动的R-R间隔之间的线性回归线的斜率。对含3个以上间隔的序列， 0.5mmHg的BP变化，以及5ms的R-R间隔变化进行分析。所有这些斜率的平均值作为BRS。 在时间和频率域里分析了HRV（心率变异性）。时域方法，我们采用连续时间间隔的平均平方差的平方根，这反映了HR的短期变化。采用快速傅里叶变换计算收缩压和R-R间隔时间序列的功率频谱（段长512跳动，12Hz重新采样）以及交差谱。压力感受器反射的增益被定为在低频（LF）和高频（HF）段转移函数的平均值。分析老鼠时考虑到HR的范围和呼吸频率,频率段进行了调整（极低频率LF=0.015至0.25Hz, LF=0.25至1Hz, LF=1至6Hz,）。如果分析频率段的相关性系数大于0.8，则认为BRS非常重要。数据分析是采用了PV波软件 |
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