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[资源] Calcium-Binding Protein Protocols (Vol 2) (Methods in Molec Bio pdf

Calcium-Binding
Protein
Protocols
Volume 2: Methods and Techniques
Edited by
Hans J. Vogel
Department of Biological Sciences, University of Calgary
Calgary, AB, Canada
Contents
Dedication .........................................................................................................v
Preface ........................................................................................................... vii
Contents of Companion Volume ................................................................... xiii
Contributors..................................................................................................xv
PART III. METHODS AND TECHNIQUES TO STUDY
CALCIUM-BINDING PROTEINS
1 Quantitative Analysis of Ca2+-Binding by Flow Dialysis
Michio Yazawa ........................................................................................ 3
2 Calcium Binding to Proteins Studied via Competition
with Chromophoric Chelators
Sara Linse ............................................................................................. 15
3 Deconvolution of Calcium-Binding Curves: Facts and Fantasies
Jacques Haiech and Marie-Claude Kilhoffer .................................... 25
4 Absorption and Circular Dichroism Spectroscopy
Stephen R. Martin and Peter M. Bayley ............................................ 43
5 Fourier Transform Infrared Spectroscopy
of Calcium-Binding Proteins
Heinz Fabian and Hans J. Vogel ........................................................ 57
6 Steady-State Fluorescence Spectroscopy
Aalim M. Weljie and Hans J. Vogel .................................................... 75
7 Fluorescence Methods for Measuring Calcium Affinity and Calcium
Exchange with Proteins
J. David Johnson and Svetlana B. Tikunova ................................... 89
8 Surface Plasmon Resonance of Calcium-Binding Proteins
Karin Julenius .................................................................................... 103
9 Differential Scanning Calorimetry
Maria M. Lopez and George I. Makhatadze .................................... 113
10 Isothermal Titration Calorimetry
Maria M. Lopez and George I. Makhatadze .................................... 121
11 Multiangle Laser Light Scattering and Sedimentation Equilibrium
Leslie D. Hicks, Jean-René Alattia, Mitsuhiko Ikura,
and Cyril M. Kay ............................................................................. 127
x Contents
12 Small-Angle Solution Scattering Reveals Information
on Conformational Dynamics in Calcium-Binding Proteins
and in their Interactions with Regulatory Targets
Jill Trewhella and Joanna K. Krueger ............................................. 137
13 Investigation of Calcium-Binding Proteins Using Electrospray
Ionization Mass Spectrometry
Amanda L. Doherty-Kirby and Gilles A. Lajoie .............................. 161
14 Synthetic Calcium-Binding Peptides
Gary S. Shaw ...................................................................................... 175
15 Proteolytic Fragments of Calcium-Binding Proteins
Richard D. Brokx and Hans J. Vogel ............................................... 183
16 Electron Magnetic Resonance Studies
of Calcium-Binding Proteins
Lawrence J. Berliner ......................................................................... 195
17 Cadmium-113 and Lead-207 NMR Spectroscopic Studies
of Calcium-Binding Proteins
Teresa E. Clarke and Hans J. Vogel ................................................ 205
18 Calcium-43 of NMR of Calcium-Binding Proteins
Torbj?rn Drakenberg ......................................................................... 217
19 Exploring Familial Relationships Using Multiple
Sequence Alignment
Aalim M. Weljie and Jaap Heringa ................................................... 231
20 Structure Determination by NMR: Isotope Labeling
Monica X. Li, David C. Corson, and Brian D. Sykes ...................... 255
21 Protein Structure Calculation from NMR Data
Tapas K. Mal, Stefan Bagby, and Mitsuhiko Ikura ........................ 267
22 Shape and Dynamics of a Calcium-Binding Protein Investigated
by Nitrogen-15 NMR Relaxation
J?rn M. Werner, Iain D. Campbell, and A. Kristina Downing ....... 285
23 The Use of Dipolar Couplings for the Structure Refinement
of a Pair of Calcium-Binding EGF Domains
Jonathan Boyd, Iain D. Campbell, and A. Kristina Downing ....... 301
24 Vector Geometry Mapping: A Method to Characterize
the Conformation of Helix-Loop-Helix Calcium-Binding Proteins
Kyoko L. Yap, James B. Ames, Mark B. Swindells,
and Mitsuhiko Ikura ...................................................................... 317
25 Use of Calmodulin Antagonists and S-100 Protein Interacting
Drugs for Affinity Chromatography
Ryoji Kobayashi ................................................................................. 325
26 Enzymatic Assays to Compare Calmodulin Isoforms, Mutants,
and Chimeras
Michael P. Walsh, Jacquelyn E. Van Lierop, Cindy Sutherland,
Ritsu Kondo, and J. David Johnson ........................................... 339
27 Gene Expression in Transfected Cells
Kate Hughes, Juha Saarikettu, and Thomas Grundstr?m ........... 355
28 Monitoring the Intracellular Free Ca2+-Calmodulin Concentration
with Genetically-Encoded Fluorescent Indicator Proteins
Anthony Persechini ........................................................................... 365
29 Studying the Spatial Distribution of Ca2+-Binding Proteins:
How Does it Work for Calmodulin?
Katalin T?r?k, Richard Thorogate, and Steven Howell ................ 383
Index ............................................................................................................ 409

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