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天使托

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dhd997(金币+5, MolEPI+1): good 2011-08-24 10:55:56
Whenever,Wherever,Anyway!MustRemember:Fight!KeepFighting!NeverGiveUp!
2楼2011-08-24 09:55:00
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labrat

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1949stone(MolEPI+1): 学习下 2011-08-24 20:14:08
sunyueyan(金币+1): 谢谢回帖 2011-08-30 09:28:01
It is a DNA Sequencing Methods.
Pls refer textbooks or references for more details.
Primer walking is a step-by-step approach to sequencing long DNA templates from end to end that overcomes the inability of the Sanger chain termination method to read more than a few hundred bases in a single reaction. After an initial round of sequencing from a known sequence at one end of the template, each subsequent round is initiated from a new primer, which is based on the end of the sequence obtained from the previous reaction.

Keywords: primer walking; DNA sequencing; genome; gap closure; hexamer library

Ref: http://www.els.net/WileyCDA/ElsArticle/refId-a0005382.html

Primer Walking in the Finishing Lab at the Broad Institute; This reference give you an general idea of what it is.
Ref: http://www.lanl.gov/conferences/ ... %2520for%2520NM.pdf
3楼2011-08-24 12:10:34
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