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liping515110

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[求助] 求高手帮忙润色一下我的英语翻译

尽管目前把淡水纤毛虫、水螅和海绵共生绿藻暂归类于小球藻属（genus Chlorella），但是我们对共生绿藻的物种多样性和这些藻类的宿主分布仍然是知之甚少。但是，Kessler（1984）和Reisser等（1988）的初步研究表明共生小球藻的遗传多样性可能超乎想像，但总的来说，许多类似研究皆未能阐明来源不同的共生小球藻的种间关系（Huss等1989）。Reisser等（1988）鉴定的共生小球藻Chlorella vulgaris与Huss等（1990）鉴定分析的小球藻Chlorella vulgaris的遗传实体是否一样现在不得而知。另外产自英国的绿水螅Hydra sp.的共生绿藻和产自美国佛罗里达的绿水螅Hydra sp.的共生小球藻可能为不同种的小球藻，这也可能因为二者的宿主绿水螅根本不是同种（Muscatine et al.，1975；Douglas et al.，1984）。简而言之，同一宿主的不同地理种群所共生的绿藻是否为同种、不同宿主的共生绿藻能否有可能为同种，这些问题的解答还有待于对共生绿藻起源和进化深层次的研究。
本研究尝试基于中国绿水螅及GenBank中现有的共生藻的核18S rRNA和ITS基因片段序列和小球藻属所在的Trebouxiophyceae纲的主要非共生的、自由生活的绿藻代表物种的同源序列构建分子系统发生树，期待初步探讨不同来源的共生藻与Trebouxiophyceae纲中自由生活的绿藻之间的系统发生关系，从而证实共生藻是多系起源还是单系起源。目前不同来源的共生藻核18S rRNA序列稍显丰富，所以基于共生绿藻和Trebouxiophyceae纲中自由生活的绿藻核18S rRNA序列的系统发生树（图3-2，图3-3，图3-4）给我们提供较为丰富的遗传信息。基于18S rRNA基因序列重建的ML树（图3-2）、MP树（图3-3）和Bayesian树（图3-4）均表明，参与建树的Trebouxiophyceae纲的61个种明显分离为2大分支，一个分支为共生绿藻支系，其中有水螅共生绿藻、草履虫共生绿藻、银杏共生绿藻及苔藓共生绿藻夹杂其中，而另一分支为无共生绿藻支系。这个结果表明从Trebouxiophyceae纲的大框架来看，不同来源的共生绿藻似乎有一共同起源；但从共生绿藻支系的小框架来看，不同来源的共生藻并没有排他性地聚为单系群，而在系统树中与其他自由生活的绿藻混杂排列，不同来源的共生绿藻似乎没有一共同起源。
Although the endosymbiotic green algae from freshwater ciliates, hydra or sponges were all attributed to genus Chlorella at present, we still know little about the species diversity and host distribution of them. However, the preliminary studies from Kessler(1984) and Reisser(1988) indicate that the genetic diversity of symbiotic Chlorella may be unimaginable,but in general, many similar researchs all
failed to clarify the interspecies relationships of symbiotic Chlorella from different sources(Huss et al, 1989).It is still unable to find out that whether the symbiotic Chlorella vulgaris inentified by Reisser (1988) and the Chlorella valgaris identified by Huss(1990) have the same genetic entity or not. In addition,the symbiotic green algae of Hydra sp.obtained from UK and the symbiotic Chlorella of Hydra sp. produced in Florida may be two different Chlorella species,which may because that the two host hydras are not the same species at all.In brief,whether the symbiotic algae from the same host of different geographic populations are the same species or not and whether the symbiotic algae from different hots may be the same species or not, to answer these questions ,we need a futher deep research in the origin and evolution of the symbiotic algae.
This study attempts to construct molecular phylogenetic trees based on the nuclear 18S rRNA and ITS gene sequences of the symbiotic algae obtained from hydra sinensis and GenBank and the homologous sequences of the representative species from non-symbiotic、free-living green algae within Chlorella genus that belongs to Class Trebouxiophyceae,looking forward to give a preliminary discussion about phylogenetic relationships between the symbiotic algae from different sources and free-living algae in Class Trebouxiophyceae,thus confirming whether the origin of symbiotic algae was polyphyletic or monophyletic.Currently, 18S rRNA sequences of symbiotic algae from difference sources are abundant, so phylogenetic trees(figure3-2,figue3-3,figue3-4) based on 18S rRNA sequences of symbiotic algae ,and free-living green algae within Class Trebouxiophyceae, provides a wealth of genetic information for us. The ML(figure3-2)、MP(figure3-3) and Bayesian trees(figure3-4) based on 18S rRNA gene sequences all indicated that 61 Species of Class Trebouxiophyceae was separated into two branches, one branch was symbiotic algae group which consist of endosymbiotic algae from hydra, paramecium, ginkgo and moss and many free-living algae, and other branch was free-living algae group which consist only of free-living algae. The result suggests that，from the perspective of Class Trebouxiophyceae broad framework, the symbiotic algae from different sources seem to have a common origin; but from the perspective of symbiotic algae group small framework, Symbiotic algae from different sources does not form a monophyletic group exclusively, but mixed with other free-living algae in phylogenetic trees, they do not seem to have a common origin.
以上是我的英语翻译前面是中文求高手改改谢谢！！！

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1楼2011-08-07 22:17:11

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phu_grassman

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爱与雨下(金币+2): 感谢应助 2011-08-08 14:47:46
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引用回帖:

1楼: Originally posted by liping515110 at 2011-08-07 22:17:11:
尽管目前把淡水纤毛虫、水螅和海绵共生绿藻暂归类于小球藻属（genus Chlorella），但是我们对共生绿藻的物种多样性和这些藻类的宿主分布仍然是知之甚少。但是，Kessler（1984）和Reisser等（1988）的初步研究表明 ...

非专业人士，专业术语什么的没加修改，希望对你有帮助你，同时也希望大家多多指正。
Although the endosymbiotic green algae from freshwater ciliates, hydra or sponges were(are) all attributed to genus Chlorella at present, we still know little about the species diversity and host distribution of them(know little about their species diversity and host distribution). However, the preliminary studies from Kessler(1984) and Reisser(1988) indicate(indicated) that the genetic diversity of symbiotic Chlorella may be unimaginable(beyond imagination 或者我认为这样表达the symbiotic Chlorella had an unexpected genetic diversity),but in general, many similar researchs all failed to clarify the interspecies relationships of symbiotic Chlorella from different sources(Huss et al, 1989)（这个句子为什么这么复杂呢？）.It is still unable to find out thatwhether the symbiotic Chlorella vulgaris inentified by Reisser (1988) and the Chlorella valgaris identified by Huss(1990) have the same genetic entity or not（still unknown whether the genetic entity of the symbiotic Chlorella vulgaris identified by Reisser (1988) is the same as that of the Chlorella valgaris identified by Huss(1990)）. In addition,the symbiotic green algae of Hydra sp.obtained from UK and the symbiotic Chlorella（that代替the symbiotic Chlorella） of Hydra sp. produced in Florida may be two different Chlorella species（belong to two different species）,which may because that the two host hydras are not the same species at all(照中文应该是which may also be the same species（Muscatine et al.，1975；Douglas et al.，1984）).In brief, whether the symbiotic algae from the same host of different geographic populations are the same species or not and whether the symbiotic algae from different hots may be the same species or not, to answer these questions ,we need a futher deep research in the origin and evolution of the symbiotic algae.(we still need further study on the orign and evolution of the symbiotic algae to ask the questions of whether the symbiotic algae from the same host but different geographic populations are the same species and whether the symbiotic algae from different hosts are the same species.)
This study attempts(attempted) to construct molecular phylogenetic trees based on the nuclear 18S rRNA and ITS gene sequences of the symbiotic algae obtained from hydra sinensis and GenBank and the homologous sequences of the representative species from non-symbiotic、free-living green algae within Chlorella genus that belongs to Class Trebouxiophyceae,(不得不说，这个句子真的好复杂！based on the gene sequences of nuclear 18S rRNA and ITS of the existing symbiotic algae obtained from hydra sinensis in the hydra sinensis and GenBank and the main non-symbiotic homologous sequences of the Class Trebouxiophyceae within the Chlorella genus),looking forward to give a preliminary discussion（expecting a preliminary discussion） about (the) phylogenetic relationships between the symbiotic algae from different sources and the free-living algae in Class Trebouxiophyceae, thus confirming whether the origin of symbiotic algae was polyphyletic or monophyletic. Currently, 18S rRNA sequences of symbiotic algae from difference sources are abundant, so phylogenetic trees(figure3-2,figue3-3,figue3-4) based on 18S rRNA sequences of symbiotic algae ,and free-living green algae within Class Trebouxiophyceae, provides(provide) a wealth of genetic information for us. The ML(figure3-2)、MP(figure3-3) and Bayesian trees(figure3-4) based on 18S rRNA gene sequences all indicated that 61 Species of Class Trebouxiophyceae was(were) separated into two branches, one branch was (the) symbiotic algae group which consist of endosymbiotic algae from hydra, paramecium, ginkgo and moss and many free-living algae, and (the) other branch was free-living algae group which consist only(only consist) of free-living algae. The result suggests that，from the perspective of Class Trebouxiophyceae broad framework, the symbiotic algae from different sources seem to have a common origin(suggests the symbiotic algae from different sources seems to have a common origin from the perspective of the framework of the Class Trebouxiophyceae ); but from the perspective of symbiotic algae group small framework, Symbiotic algae from different sources does not form a monophyletic group exclusively, but mixed(mixes) with other free-living algae in phylogenetic trees, they do not seem to have a common origin.