| 查看: 4232 | 回复: 101 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[资源]
分享一篇讨论反相液相色谱流动相PH的综述
|
||
|
On the Effect of Organic Solvent Composition on the pH of Buffered HPLC Mobile Phases and the pK(a) of Analytes - A Review A review about the analyte pK a and buffer pH variations in RP‐HPLC mobile phases with the changes in the organic modifier content (acetonitrile or methanol) is presented. A model to accurately predict the pH of particular mobile phases for several commonly used buffers (acetic, citric and phosphoric acid and ammonia systems) in acetonitrile‐water and methanol‐water mixtures is described. Linear relationships are also presented for several families of acid‐base compounds (aromatic and aliphatic carboxylic acids, phenols, amines and pyridines) to estimate pK a values of analytes in methanol‐water and acetonitrile‐water from their corresponding aqueous pK a. From both, the estimated pH of the mobile phase and the estimated pK a of acid‐base analytes, it is possible to predict their degree of ionization and, therefore, the analyte chromatographic retention.[ Last edited by cat-liujiang on 2011-8-4 at 16:35 ] |
回复此楼» 猜你喜欢
面上项目申报
已经有3人回复
-
有时候真觉得大城市人没有县城人甚至个体户幸福
已经有5人回复
-
酰胺脱乙酰基
已经有9人回复
-
CSC & MSCA 博洛尼亚大学能源材料课题组博士/博士后招生｜MSCA经费充足、排名优
已经有5人回复
-
博士延得我，科研能力直往上蹿
已经有7人回复
-
退学或坚持读
已经有27人回复
-
面上基金申报没有其他的参与者成吗
已经有5人回复
-
遇见不省心的家人很难过
已经有22人回复
顶一下 非常实用 54楼2013-04-17 00:13:57
简单回复
2011-08-04 16:38
回复
三星好评 顶一下，感谢分享！
绿杨烟外4楼
2011-08-04 16:57
回复
五星好评 顶一下，感谢分享！
2011-08-06 10:43
回复
五星好评 顶一下，感谢分享！
陈林霖6楼
2011-08-06 13:34
回复
三星好评 顶一下，感谢分享！
cxgxb9楼
2011-10-01 00:37
回复
三星好评 
bozi198811楼
2011-11-05 19:00
回复
五星好评 顶一下，感谢分享！
lvxingan198912楼
2012-03-05 21:23
回复
三星好评 顶一下，感谢分享！
罗小赛14楼
2012-03-06 22:28
回复
五星好评 顶一下，感谢分享！