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[资源]
分享一篇讨论反相液相色谱流动相PH的综述
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On the Effect of Organic Solvent Composition on the pH of Buffered HPLC Mobile Phases and the pK(a) of Analytes - A Review A review about the analyte pK a and buffer pH variations in RP‐HPLC mobile phases with the changes in the organic modifier content (acetonitrile or methanol) is presented. A model to accurately predict the pH of particular mobile phases for several commonly used buffers (acetic, citric and phosphoric acid and ammonia systems) in acetonitrile‐water and methanol‐water mixtures is described. Linear relationships are also presented for several families of acid‐base compounds (aromatic and aliphatic carboxylic acids, phenols, amines and pyridines) to estimate pK a values of analytes in methanol‐water and acetonitrile‐water from their corresponding aqueous pK a. From both, the estimated pH of the mobile phase and the estimated pK a of acid‐base analytes, it is possible to predict their degree of ionization and, therefore, the analyte chromatographic retention.[ Last edited by cat-liujiang on 2011-8-4 at 16:35 ] |
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顶一下 非常实用54楼2013-04-17 00:13:57
简单回复
2011-08-04 16:38
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三星好评 顶一下，感谢分享！
绿杨烟外4楼
2011-08-04 16:57
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五星好评 顶一下，感谢分享！
2011-08-06 10:43
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五星好评 顶一下，感谢分享！
陈林霖6楼
2011-08-06 13:34
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三星好评 顶一下，感谢分享！
cxgxb9楼
2011-10-01 00:37
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三星好评 
bozi198811楼
2011-11-05 19:00
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五星好评 顶一下，感谢分享！
lvxingan198912楼
2012-03-05 21:23
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三星好评 顶一下，感谢分享！
罗小赛14楼
2012-03-06 22:28
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五星好评 顶一下，感谢分享！