| 查看: 4642 | 回复: 101 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[资源]
分享一篇讨论反相液相色谱流动相PH的综述
|
||
|
On the Effect of Organic Solvent Composition on the pH of Buffered HPLC Mobile Phases and the pK(a) of Analytes - A Review A review about the analyte pK a and buffer pH variations in RP‐HPLC mobile phases with the changes in the organic modifier content (acetonitrile or methanol) is presented. A model to accurately predict the pH of particular mobile phases for several commonly used buffers (acetic, citric and phosphoric acid and ammonia systems) in acetonitrile‐water and methanol‐water mixtures is described. Linear relationships are also presented for several families of acid‐base compounds (aromatic and aliphatic carboxylic acids, phenols, amines and pyridines) to estimate pK a values of analytes in methanol‐water and acetonitrile‐water from their corresponding aqueous pK a. From both, the estimated pH of the mobile phase and the estimated pK a of acid‐base analytes, it is possible to predict their degree of ionization and, therefore, the analyte chromatographic retention.[ Last edited by cat-liujiang on 2011-8-4 at 16:35 ] |
回复此楼» 猜你喜欢
有多少人是今天查系统知道结果的？
已经有17人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.54,科目齐全,可+急
已经有4人回复
-
基金不中，共勉
已经有5人回复
-
看板上这么多中的，有点像50人群里49个人都是骗子的那种感觉……
已经有3人回复
-
国自然面上复盘~欢迎讨论
已经有5人回复
-
我不理解！
已经有23人回复
-
2026年的国家社科基金项目通讯评审的新规则与新动向、新挑战
已经有10人回复
-
系统查不到
已经有10人回复
-
怎么查啊
已经有5人回复
-
范进中举一文的中心思想
已经有10人回复
顶一下 非常实用 54楼2013-04-17 00:13:57
简单回复
2011-08-04 16:38
回复
三星好评 顶一下，感谢分享！
绿杨烟外4楼
2011-08-04 16:57
回复
五星好评 顶一下，感谢分享！
2011-08-06 10:43
回复
五星好评 顶一下，感谢分享！
陈林霖6楼
2011-08-06 13:34
回复
三星好评 顶一下，感谢分享！
cxgxb9楼
2011-10-01 00:37
回复
三星好评 
bozi198811楼
2011-11-05 19:00
回复
五星好评 顶一下，感谢分享！
lvxingan198912楼
2012-03-05 21:23
回复
三星好评 顶一下，感谢分享！
罗小赛14楼
2012-03-06 22:28
回复
五星好评 顶一下，感谢分享！
投票:
