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| Several of these protocols have been developed to incorporate a nucleic acid purification step involving a solid supportmatrix with an affinity for nucleic acid (Hong et al. 2003) or an affinity for select nucleic acid species (polyadenylated mRNA) (Kim et al. 2008) or target sequences (Regan &Margolin 1997). Once bound, the nucleic acid can be washed free of the PCR inhibitors and eluted from the spin columns (Luan & Levin 2008) or beads (Hong et al. 2003, Kim et al. 2008) used to concentrate the PCR template. The physiochemical properties of foods can also be exploited by altering the temperature (Hong et al. 2003), using differential or density gradient centrifugation (Hong et al. 2003, Lindqvist 1997, Maurer et al. 1999, Wolffs et al. 2004, 2007), or using filtration (Butot et al. 2007, Wolffs et al. 2006) to remove fats (Hong et al. 2003, Maurer et al. 1999), colloids (Maurer et al. 1999), blood (Hong et al. 2003), or tissues (Butot et al. 2007). These procedures can effectively concentrate the template as well as remove PCR inhibitors. Density gradient centrifugation has the broadest application toward PCR detection of pathogens across diverse food matrices (Lindqvist 1997, Wolffs et al. 2004, 2007). However, some foods may necessitate extractions or digestions (Butot et al. 2007) to ultimately free the template of these inhibitors. Application of activated charcoal to samples has also proven effective in removing PCR inhibitors (Luan & Levin 2008). |
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5楼2011-07-07 20:22:20
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至尊木虫 (职业作家)
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2楼2011-07-05 09:06:30
clzkzy
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3楼2011-07-05 09:59:38
LittleBush
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sltmac(金币+2): 说的很不错~ 2011-07-06 08:15:32
sltmac(金币+2): 说的很不错~ 2011-07-06 08:15:32
| I totally agree with the opinions made by the above two.This posted requested translation is very easy to understand and it is just very common knowledge in the PCR technique.If you have difficulty in understanding such materials,I strongly suggest that you should make up for your English at once.In this globalized era，a graduate student or a research who have not good command of the English language will have no chance to win in his career because the newest and most important advances in nearlly every displine are written in English .Then recognition of its significance，you must make the most determination to learn it well.Indeed,there is no shortcut and you must learn it every day.No pain ,no gain.Good luck. |
4楼2011-07-05 11:39:31
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