| 查看: 925 | 回复: 5 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[求助]
在线等高手翻译
|
||
| Several of these protocols have been developed to incorporate a nucleic acid purification step involving a solid supportmatrix with an affinity for nucleic acid (Hong et al. 2003) or an affinity for select nucleic acid species (polyadenylated mRNA) (Kim et al. 2008) or target sequences (Regan &Margolin 1997). Once bound, the nucleic acid can be washed free of the PCR inhibitors and eluted from the spin columns (Luan & Levin 2008) or beads (Hong et al. 2003, Kim et al. 2008) used to concentrate the PCR template. The physiochemical properties of foods can also be exploited by altering the temperature (Hong et al. 2003), using differential or density gradient centrifugation (Hong et al. 2003, Lindqvist 1997, Maurer et al. 1999, Wolffs et al. 2004, 2007), or using filtration (Butot et al. 2007, Wolffs et al. 2006) to remove fats (Hong et al. 2003, Maurer et al. 1999), colloids (Maurer et al. 1999), blood (Hong et al. 2003), or tissues (Butot et al. 2007). These procedures can effectively concentrate the template as well as remove PCR inhibitors. Density gradient centrifugation has the broadest application toward PCR detection of pathogens across diverse food matrices (Lindqvist 1997, Wolffs et al. 2004, 2007). However, some foods may necessitate extractions or digestions (Butot et al. 2007) to ultimately free the template of these inhibitors. Application of activated charcoal to samples has also proven effective in removing PCR inhibitors (Luan & Levin 2008). |
回复此楼» 猜你喜欢
085404 298分求调剂
已经有6人回复
-
有爆料，一个青年教师卖房得400万，然后换了一个四青帽子
已经有9人回复
-
求调剂
已经有9人回复
-
求调剂 材料与工程 324分 专硕
已经有21人回复
-
0858求调剂
已经有7人回复
-
化学工程与技术324调剂
已经有24人回复
-
求调剂
已经有8人回复
-
电子信息279求调剂，有书读就行
已经有7人回复
-
311求调剂
已经有10人回复
-
人工智能320调剂08工类还有机会吗
已经有3人回复
5楼2011-07-07 20:22:20
8814402
至尊木虫 (职业作家)
- 翻译EPI: 509
- 应助: 18 (小学生)
- 贵宾: 0.381
- 金币: 12916.1
- 散金: 47
- 红花: 16
- 帖子: 4183
- 在线: 357.8小时
- 虫号: 1184404
- 注册: 2011-01-06
- 专业: 药物学其他科学问题
2楼2011-07-05 09:06:30
clzkzy
木虫 (正式写手)
小二黑
- 翻译EPI: 23
- 应助: 29 (小学生)
- 金币: 2050.6
- 散金: 543
- 红花: 14
- 帖子: 913
- 在线: 798.2小时
- 虫号: 941199
- 注册: 2010-01-12
- 性别: GG
- 专业: 电化学分析
3楼2011-07-05 09:59:38
LittleBush
至尊木虫 (著名写手)
- 翻译EPI: 15
- 应助: 0 (幼儿园)
- 金币: 30705.8
- 红花: 32
- 帖子: 1348
- 在线: 712.4小时
- 虫号: 107112
- 注册: 2005-11-17
- 性别: GG
- 专业: 数论
★ ★
sltmac(金币+2): 说的很不错~ 2011-07-06 08:15:32
sltmac(金币+2): 说的很不错~ 2011-07-06 08:15:32
| I totally agree with the opinions made by the above two.This posted requested translation is very easy to understand and it is just very common knowledge in the PCR technique.If you have difficulty in understanding such materials,I strongly suggest that you should make up for your English at once.In this globalized era，a graduate student or a research who have not good command of the English language will have no chance to win in his career because the newest and most important advances in nearlly every displine are written in English .Then recognition of its significance，you must make the most determination to learn it well.Indeed,there is no shortcut and you must learn it every day.No pain ,no gain.Good luck. |
4楼2011-07-05 11:39:31
