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100817032107铜虫 (小有名气)
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[求助]
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DAS-QSO-INAH (1.001 g) was added to 50 ml of a l/15 M phosphate buffer, pH 7.4 which was prepared with D,O instead of H,O. This suspen- sion was stirred constantly at 1000 rpm at 37°C. Each aliquot sample (5 ml) was withdrawn after 3, 8, 13,18, 23 and 28 days and replaced by the same volume of the dissolution fluid. After 3 ml was taken from each sample and freeze-dried, the total released content of INAH was calculated from the weight and its contained nitrogen ratio. The property of INAH skelton release was checked by NMR under the similar condition where DAS-QSO-INAH (2.004 g) stirred in the above phosphate buffer, pH 7.4 (100 ml) at 1000 rpm and at 37°C. Aliquot samples (5 ml) were withdrawn at 3, 6, 9, 12, 15 and 33 days and the buffer (5 ml) was supplied after each sampling. D,O buffer (0.2 ml) containing methanol (0.79 mg) was added as a standard in 1 ml of each sample. The molar ratio of INAH skelton protons to methyl protons of methanol gave the content of INAH skeltons. However, this calculated value was used for comparison with the estimation by weight and nitrogen ratio because it was difficult to identify released compounds completely. The released compounds were estimated by employing the TLC method following a similar operation in which DAS-QSO-INAH (400 mg) was added to 20 ml of a l/15 M phosphate buffer, pH 7.4 and stirred at 1000 rpm at 37°C. Aliquot samples were withdrawn at the appropriate time intervals and used for TLC, while the same volume of dissolution fluid was supplied after each sampling. INAH (2 mg/ml), INA (2.1 mg/ml) and INAA (2.1 mg/ml) were prepared as standards. Ten drops of all samples were placed on a TLC plate with 2/l (v/v) C,H,0H/CHC13. Concerning intact INAH, a similar experiment was carried out under the same conditions except that INAH (400 mg) was used instead of DAS-QSO-INAH (400 mg). Aliquot samples were checked by TLC. The fluorescence technique and ninhydrin reaction were used for detection. |
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8814402
至尊木虫 (职业作家)
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2楼2011-06-29 08:47:27
8814402
至尊木虫 (职业作家)
- 翻译EPI: 509
- 应助: 18 (小学生)
- 贵宾: 0.381
- 金币: 12916.1
- 散金: 47
- 红花: 16
- 帖子: 4183
- 在线: 357.8小时
- 虫号: 1184404
- 注册: 2011-01-06
- 专业: 药物学其他科学问题
3楼2011-06-29 08:51:25
