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dandan_lan金虫 (初入文坛)
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[求助]
干细胞方向的，帮我翻译一下行吗？
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While researchers have a viable means to correct the mutation in these iPS cells, other steps in the development of patient-specific therapeutic stem cells remain problematic. For example, recent studies have found that many mutations arise during reprogramming of somatic cells into iPS cells, a finding that Howden’s team confirmed in their study. To develop the tissue-specific regenerative capacity of the patient’s cell, researchers rewound the cell into a pluripotent state by overexpressing seven proteins—OCT4, SOX2, NANOG, LIN28, c-Myc, KLF4, and SV40 T-antigen—whose coding sequences reside on an episomal DNA. “We used the episomal reprogramming methods because the genes carried on episomes are extra-chromosomal which means that they don’t integrate into the chromosome,” Howden says. This reprogramming method left the genome intact. Now Howden and colleagues are obtaining biopsy samples from patients who have an autosomal dominant form of retinal dysfunction, hoping to continue reducing the number of mutations in the development of stem cells for clinical applications. The paper, “Genetic correction and analysis of induced pluripotent stem cells from a patient with gyrate atrophy,” was published 1 March 2011 in Proceedings of the National Academy of Sciences. |
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zuodg
铁杆木虫 (正式写手)
有为青年
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【答案】应助回帖
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Mally89(金币+2): 翻译的就是好~~~O(∩_∩)O~ 2011-06-17 20:27:49
dandan_lan(金币+30, 翻译EPI+1): 谢谢你！ 2011-06-21 10:43:07
Mally89(金币+2): 翻译的就是好~~~O(∩_∩)O~ 2011-06-17 20:27:49
dandan_lan(金币+30, 翻译EPI+1): 谢谢你！ 2011-06-21 10:43:07
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虽然研究者能够修正多能肝细胞的突变，然而病人专一性治疗肝细胞在发育过程中仍然困难重重。例如，Howden的研究发现，体细胞重新编程生成多能肝细胞的过程中会发生很多突变。 为了使病人细胞获得组织专一性再生能力，研究者表达7个蛋白，即OCT4, SOX2, NANOG, LIN28, c-Myc, KLF4, and SV40 T-抗体（编码序列在附加体DNA），使细胞出现多能状态。 Howden说，“我们使用附加体重编程技术，是因为附加体上面的基因是染色体外的基因，不与染色体发生整合。” 该技术不会破坏基因组的完整性。 现在Howden及同事从常染色体显性遗传形式的视网膜功能障碍患者身上获得了活检样品，希望能够继续减少干细胞发育过程中突变的数量。 这篇文章“Genetic correction and analysis of induced pluripotent stem cells from a patient with gyrate atrophy”发表在2011年3月1日的PNAS杂志上。 |
2楼2011-06-17 13:40:55
